Effects of bFGF incorporated into a gelatin sheet on wound healing
Effects of bFGF incorporated into a gelatin sheet on wound healing
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DOI：
10.1163/1568562054255709
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发表时间：
2005-01-01
影响因子：
3.6
通讯作者：
Suzuki, S
中科院分区：
文献类型：
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作者：
Miyoshi, M;Kawazoe, T;Suzuki, S
众所周知，碱性成纤维细胞生长因子（bFGF）促进几乎所有与伤口愈合相关的细胞的增殖。然而，由于碱性成纤维细胞生长因子在体内的激活时间很短，我们将碱性成纤维细胞生长因子纳入酸性明胶水凝胶，并研究了碱性成纤维细胞生长因子在体内的持续释放。此外，我们研究了含bFGF的酸性明胶片对伤口愈合的影响。为了区分伤口收缩与新上皮形成，我们测量了伤口面积和新上皮长度。其他组织学参数如肉芽组织厚度和毛细血管数量也被确定为伤口愈合的指标。使用Elastica货车Gieson和Azan染色评估纤维组织。在每只试验小鼠的背部产生全厚度的皮肤缺损（1.5 × 1.5 cm），并用酸性明胶水凝胶（在本研究中称为明胶片（2 × 2 cm））覆盖伤口，其中含有bFGF（100 μ g/部位）（A）或不含bFGF（B）。覆盖后1、2、3、5、7和14天，处死小鼠，进行酶联免疫吸附测定（ELISA）以估计血浆中bFGF的浓度。在另一个实验中，用（A）、（B）或水凝胶敷料（对照组，C）覆盖每个伤口，并在术后1或2周用计算机面积计测量伤口面积。如上所述，使用光学显微镜评估组织学参数。观察到bFGF从明胶片持续释放，并且含有bFGF的明胶片促进新上皮形成、肉芽形成、新血管形成和伤口闭合。结论：这种含有bFGF的明胶片对伤口愈合有效，具有临床应用前景。
Basic fibroblast growth factor (bFGF) is well known to promote the proliferation of almost all cells associated with wound healing. However, as the activation duration of bFGF is very short in vivo, we incorporated bFGF into an acidic gelatin hydrogel and studied the sustained release of bFGF in vivo. In addition, we investigated the effects of the acidic gelatin sheet containing bFGF on wound healing. To distinguish wound contraction from neoepithelialization, we measured both the wound area and neoepithelium length. Other histological parameters such as thickness of granulation tissue and number of capillaries were also determined as indices of wound healing. Fibrous tissue was assessed using an Elastica van Gieson and Azan stain. A skin defect (1.5 x 1.5 cm) of full thickness was created on the back of each test mouse and the wound was covered with an acidic gelatin hydrogel, referred to as a gelatin sheet in this study (2 x 2 cm), with bFGF (100 mu g/site) (A) or without bFGF (B). 1, 2, 3, 5, 7 and 14 days after covering, mice were killed and an enzyme-linked immunosorbent assay (ELISA) was performed to estimate the concentration of bFGF in the plasma. In another experiment, each wound was covered with (A), (B) or a hydrogel dressing (control croup, C) and the wound area was measured 1 or 2 weeks postoperatively with a computer planimeter. The histological parameters, as mentioned above, were assessed using a light microscope. Sustained release of bFGF from the gelatin sheet was observed and the gelatin sheet containing bFGF promoted neoepithelialization, granulation, neovascularization and wound closure. This gelatin sheet containing bFGF was concluded to be effective for wound healing and promising for clinical use.