Screening serum biomarker of knee osteoarthritis using a phage display technique
Screening serum biomarker of knee osteoarthritis using a phage display technique
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DOI：
10.1016/j.clinbiochem.2011.12.018
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发表时间：
2012-03-01
影响因子：
2.8
通讯作者：
Zhong, Da
中科院分区：
文献类型：
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作者：
Weng, Xiaojun;Liao, Qiande;Zhong, Da
目的：目的：利用噬菌体随机肽库筛选膝骨关节炎（OA）的血清生物标志物，设计与方法：用纯化的膝骨关节炎患者血清免疫球蛋白G（IgG）筛选噬菌体随机肽库。以膝关节类风湿性关节炎（RA）、髋关节OA、非糜烂性手OA或糜烂性手OA患者和健康志愿者作为对照。插入肽TGLESGHGPGDS的噬菌体克隆KOA1在膝关节OA患者中的阳性率为90%，显著高于膝关节RA患者（27.8%），非糜烂性手OA患者（34.3%），糜烂型手OA患者（31.3%）和健康对照组（12.0%），而非髋关节OA患者结论：利用噬菌体随机肽库和膝关节OA患者血清筛选到的新的膝关节OA模拟肽KOA1可能是膝关节OA潜在的血清生物标志物。(C)2011年加拿大临床化学家协会。爱思唯尔公司出版All rights reserved.
Objectives: To screen serum biomarker of knee osteoarthritis (OA) using a phage random peptide library.Design and methods: A phage random peptide library of random peptide 12-mers was screened with purified immunoglobulin G (IgG) from sera of knee OA patients. Patients with knee rheumatoid arthritis (RA), hip OA, non-erosive hand OA or erosive hand OA, and healthy volunteers were used as controls.Results: A phage clone with inserted peptide TGLESGHGPGDS (named KOA1) showed 90% positive reaction rate with the knee OA patients, significantly higher than that with the knee RA patients (27.8%), the nonerosive hand OA patients (34.3%), the erosive hand OA patients (31.3%) and the healthy controls (12.0%), but not the hip OA patients (82.5%).Conclusions: The novel knee OA mimic peptide KOA1 identified with a random phage display peptide library and sera from knee OA patients could be a potential serum biomarker for knee OA. (C) 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.