Richness and diversity of bacterioplankton species along an estuarine gradient in Moreton Bay, Australia
Richness and diversity of bacterioplankton species along an estuarine gradient in Moreton Bay, Australia
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DOI：
10.1128/aem.70.6.3425-3433.2004
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发表时间：
2004-06-01
影响因子：
4.4
通讯作者：
Fuhrman, JA
中科院分区：
文献类型：
--
作者：
Hewson, I;Fuhrman, JA
对澳大利亚亚热带布里斯班河-摩顿湾河口（27°25′s, 153°5′e）浮游细菌群落多样性进行了调查。使用自动rRNA基因间间隔区分析（ARISA）研究细菌群落，该分析从混合群落DNA中扩增16S-23S核糖体DNA内部转录间隔区，并在片段分析仪上检测分离产物。样本是从整个河口和东澳大利亚海流（珊瑚海）以东的八个地点收集的。ARISA测定的浮游细菌群落的操作分类单位（OTU）丰富度在东湾站最高（S[总丰富度]= 84 ~ 85 OTU），在珊瑚海最低（S = 39 ~ 59 OTU）。丰富度与细菌丰度呈正相关；然而，多样性与盐度、NO3-和pO(4)（3-）浓度或叶绿素a浓度之间没有很强的相关性。河流站点的浮游细菌群落与海湾或珊瑚海的不同。不同站点间OTU丰富度的主要差异存在于各分类群中，每个分类群占总扩增DNA的0.1%（检测限）至0.5%，即分布的“尾部”。我们发现一些浮游细菌分类群是特定于不同的环境，而另一些浮游细菌分类群则从河流到海洋无处不在。河口浮游细菌的丰富度和多样性模式可能是生态位可用性增加、本地和邻近环境群落混合或中间干扰的结果。此外，这些结果与先前报道的其他沿海水域空间均匀的浮游细菌群落形成对比。
Bacterioplankton community diversity was investigated in the subtropical Brisbane River-Moreton Bay estuary, Australia (27degrees25'S, 153degrees5'E). Bacterial communities were studied using automated rRNA intergenic spacer analysis (ARISA), which amplifies 16S-23S ribosomal DNA internally transcribed spacer regions from mixed-community DNA and detects the separated products on a fragment analyzer. Samples were collected from eight sites throughout the estuary and east to the East Australian Current (Coral Sea). Bacterioplankton communities had the highest operational taxonomic unit (OTU) richness, as measured by ARISA at eastern bay stations (S [total richness] = 84 to 85 OTU) and the lowest richness in the Coral Sea (S = 39 to 59 OTU). Richness correlated positively with bacterial abundance; however, there were no strong correlations between diversity and salinity, NO3- and pO(4)(3-) concentrations, or chlorophyll a concentration. Bacterioplankton communities at the riverine stations were different from communities in the bay or Coral Sea. The main differences in OTU richness between stations were in taxa that each represented 0.1% (the detection limit) to 0.5% of the total amplified DNA, i.e., the "tail" of the distribution. We found that some bacterioplankton taxa are specific to distinct environments while others have a ubiquitous distribution from river to sea. Bacterioplankton richness and diversity patterns in the estuary are potentially a consequence of greater niche availability, mixing of local and adjacent environment communities, or intermediate disturbance. Furthermore, these results contrast with previous reports of spatially homogeneous bacterioplankton communities in other coastal waters.