Basonuclin in murine corneal and lens epithelia correlates with cellular maturation and proliferative ability.
Basonuclin in murine corneal and lens epithelia correlates with cellular maturation and proliferative ability.
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DOI：
10.1046/j.1432-0436.1999.6540221.x
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发表时间：
1999-12
期刊：
影响因子：
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通讯作者：
Hung Tseng;Kyoichi Matsuzaki;R. M. Lavker
中科院分区：
文献类型：
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作者：
Hung Tseng;Kyoichi Matsuzaki;R. M. Lavker
碱性核蛋白是一种锌指蛋白，组织分布高度受限。它只在层状上皮的角质形成细胞和睾丸和卵巢的生殖细胞中被大量发现。我们研究了碱性核素在小鼠角膜和晶状体上皮细胞增殖和分化中的表达模式，角膜和晶状体上皮是眼睛中两种自我更新的组织，其中包含终生增殖的细胞。用BrdU标记处于不同发育阶段的小鼠角膜和晶状体上皮细胞90 min，检测S期细胞并确定增殖率。取小鼠或大鼠全眼冷冻切片，用兔抗小鼠或兔抗人碱性核素抗体检测细胞碱性核素。在角膜上皮基底层和前赤道晶状体上皮（分别是成人角膜和晶状体上皮的增生区室）中，几乎所有细胞都表达Basonuclin。在出生后第4天，角膜上皮中的碱性核素开始表达，首先在少数细胞中表达，然后在第20天扩散到几乎所有的基底细胞。在角膜缘上皮中一致缺乏碱性核素。晶状体基底核素的检测早于角膜，局限于前赤道上皮，而在表达p57KIP2的赤道细胞中不存在，p57KIP2是这些细胞的早期分化标志物。角膜基底核蛋白和晶状体基底核蛋白的一个重要区别是前者主要是细胞核，而后者是细胞质。
Basonuclin is a zinc finger protein with highly restricted tissue distribution. It has been found in abundance only in keratinocytes of stratified epithelia and the germ cells of the testis and ovary. We studied the expression pattern of basonuclin in relation to cellular proliferation and differentiation in murine corneal and lens epithelia, two self-renewing tissues in the eye which contain cells that proliferate throughout life. Mouse corneal and lens epithelial cells at various stages of development were labeled with BrdU for 90 min to detect cells in S phase and to establish proliferative rates. Whole eyes of mouse or rat were processed for frozen sections and cellular basonuclin was detected by either a rabbit antimouse- or a rabbit anti-human-basonuclin antibody. Basonuclin was expressed in virtually all cells in the basal layer of corneal epithelium and in the pre-equatorial lens epithelium, the respective proliferative compartments of adult corneal and lens epithelia. Basonuclin expression in corneal epithelium began at post-natal life day 4, first in a few cells and then spread to virtually all basal cells at day 20. Basonuclin was consistently absent in limbal epithelium. Lens basonuclin, which was detected earlier than that of the cornea, was confined to the pre-equatorial epithelium and was absent in equatorial cells that expressed p57KIP2, an early differentiation marker for these cells. An important distinction between corneal and lens basonuclin is that the former is predominantly nuclear whereas the latter cytoplasmic.