A modified bronchoalveolar lavage procedure that allows measurement of lung epithelial lining fluid volume.
A modified bronchoalveolar lavage procedure that allows measurement of lung epithelial lining fluid volume.
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改良的支气管肺泡灌洗程序，可测量肺上皮内层液量。
DOI：
10.1164/ajrccm/141.2.314
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Cohen,AB
中科院分区：
文献类型：
--
作者：
Peterson,BT;Idell,S;MacArthur,C;Gray,LD;Cohen,AB
将标准的连续支气管肺泡灌洗技术与新的“重新冲洗”灌洗程序一起放置，以估计麻醉绵羊上皮衬里液（ELF）的体积和蛋白质浓度。将直径6.0 mm的支气管镜插入气道，对肺段进行灌洗，灌洗液中含有放射性示踪剂（高锝酸铵，nmTc04-），灌洗液滴注4个周期。通过将示踪剂浓度外推至灌洗液与ELF混合时的零时间，使示踪剂浓度下降引起的误差最小化，但没有时间受到示踪剂损失或间质液体内流的影响。在对照羊中，这些灌洗片段的ELF平均体积为1.6 ~ 1.0 ml，平均蛋白质浓度为血浆中蛋白质浓度的26 ~ 19%。升高左房压19 ~ 5 cm H20引起“心肺水肿”，对ELF容积无显著影响，但使平均蛋白浓度升高至血浆值的57 ~ 30% （p< 0.01）。肺
Wereplaced the standard serial bronchoalveolar lavage technique with a new" rewash" lavage procedure to allow estimation of the volume and protein concentration of the epithelial lining fluid (ELF) in anesthetized sheep. A bronchoscope 6.0 mm in diameter wedged in an airway was used to lavagea segment of lung with four cycles of Instillation and aspiration of the lavage solution containing a radioactive tracer (technetium pertechnetate, nmTc04-). Errors caused by the fall in concentration of the tracer during the lavage were minimized by extrapolating the tracer concentration back to time zero when the lavage solution had mixed with the ELF, but had not had time to be affected by loss of the tracer or Influx of fluid from the Interstitium. In control sheep, the ELF of these lavaged segments had a mean volume of 1.6 ą 1.0 ml and a mean protein concentration that was 26 ą 19% of the protein concentration measured in the plasma. Increasing the left atrial pressure 19 _ą 5 cm H20 to cause" cardiac lung edema" had no significant effect on the ELF volume, but it increased the mean protein concentration to 57 ą 30% of the plasma value (p< 0.01). Lung