Tissue concentration of calcium-binding protein regucalcin in rats by enzyme-linked immunoadsorbent assay
Tissue concentration of calcium-binding protein regucalcin in rats by enzyme-linked immunoadsorbent assay
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酶联免疫吸附测定大鼠钙结合蛋白调节钙素的组织浓度
DOI：
10.1007/bf00925738
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发表时间：
1993
影响因子：
4.3
通讯作者：
M. Isogai
中科院分区：
文献类型：
--
作者：
M. Yamaguchi;M. Isogai
用兔抗Rg G的酶联免疫吸附试验(ELISA)测定大鼠组织中钙结合蛋白Rg的浓度。在雄性大鼠(5周龄)中，在肝脏中，Regucalcin的含量最高。肝脏调节性钙素浓度约为0.1mgM，按调节素分子量28,800计算。μM。肾脏中的调节素水平也高于骨骼肌、十二指肠、睾丸、肺、心脏、脾、大脑皮层和海马区。同样，在雌性大鼠中，调节性钙素在肝脏中显著存在，在肾脏中仅略有出现。因此，雷古霉素在大鼠体内的组织分布具有肝脏的特异性。随着年龄的增加，大鼠肝脏中的促肾上腺皮质激素浓度也发生变化，雄性大鼠出生后5周内肝内促性腺激素水平呈线性上升，然后开始逐渐下降。这一结果与以肝脏调节素基因为探针的Northern印迹分析结果相吻合。目前的发现清楚地表明，雷古卡素是在大鼠的肝脏中特异性合成的。
The concentration of calcium-binding protein regucalcin in the tissues of rats was estimated by enzyme-linked immunoadsorbent assay (ELISA) with rabbit-anti-regucalcin IgG. In male rats (5 weeks old), regucalcin was most pronounced in the liver. Liver regulcalcin concentration was about 0.1μM, when it was calculated with regucalcin molecular weight of 28,800. The relatively higher level of regucalcin was also found in the kidney as compared with that of the skeletal muscle, duodenum, testis, lung, heart, spleen, cerebral cortex and hippocampus. Similarly in female rats, regulacalcin was remarkable in the liver, and appeared only slightly in the kidney. Thus, the tissue distribution of regucalcin in rats was specific in the liver. The concentration of regucalcin in the liver was altered with increasing age of rats; liver regucalcin level linearly increased during 5 weeks old after birth of male rats, and then began to decrease gradually. The results coincided with the previous observation of Northern blot analyses by using liver regucalcin cDNA as a probe. The present finding clearly demonstrates that regucalcin is specifically synthesized in the liver of rats.