Cyanidin-3-O-β-glucoside attenuates allergic airway inflammation by modulating the IL-4Rα-STAT6 signaling pathway in a murine asthma model
Cyanidin-3-O-β-glucoside attenuates allergic airway inflammation by modulating the IL-4Rα-STAT6 signaling pathway in a murine asthma model
复制标题
在小鼠哮喘模型中，Cyanidin-3-O-β-glucoside 通过调节 IL-4R α-STAT6 信号通路减轻过敏性气道炎症
DOI：
10.1016/j.intimp.2019.01.008
复制
发表时间：
2019-04-01
影响因子：
5.6
通讯作者：
Chen, Yanqiu
中科院分区：
文献类型：
--
作者：
Ma, Baihui;Wu, Yinfan;Chen, Yanqiu
花青素-3-O-β-葡萄糖苷（Cy-3-g）是一种典型且含量丰富的花青素单体，具有多种生物活性，如抗动脉粥样硬化、抗肥胖和抗癌作用。然而，迄今为止，人们对其对哮喘的影响知之甚少。本研究旨在探讨膳食 Cy-3-g 对动物模型过敏性哮喘的功效。 BALB/c 小鼠经卵清蛋白 (OVA) 致敏和激发以诱导过敏性哮喘。评估肺组织的病理变化、支气管肺泡灌洗液（BALF）中2型辅助细胞（Th2）相关细胞因子的产生以及白细胞介素4受体α（IL-4Rα）信号转导器和转录激活剂6（STAT6）信号通路活性。我们发现 Cy-3-g 显着抑制 OVA 诱导的肺组织炎症细胞浸润和粘液过度产生，减少 BALF 中白细胞介素 4 (IL-4)、白细胞介素 5 (IL-5) 和白细胞介素 13 (IL-13) 的产生。此外，Cy-3-g 有效抑制 OVA 诱导的肺组织 IL-4R α-STAT6 信号通路活性上调。这些结果表明，饮食中的 Cy-3-g 可以减轻小鼠哮喘模型中的过敏性气道炎症，并且 Cy-3-g 将来可能用作哮喘预防和/或治疗的药物。
Cyanidin-3-O-beta-glucoside (Cy-3-g), a typical and abundant monomer of anthocyanins, exhibits a variety of biological activities, such as anti-atherosclerosis, anti-obesity, and anticancer effects. However, to date little is known about its effects on asthma. This study aimed to investigate the efficacy of dietary Cy-3-g on allergic asthma in an animal model. BALB/c mice were sensitized and challenged with ovalbumin (OVA) to induce allergic asthma. The pathological changes of the lung tissues, type 2 helper (Th2)-associated cytokine production in bronchoalveolar lavage fluid (BALF), and the interleukin 4 receptor alpha (IL-4R alpha)-signal transducer and activator of transcription 6 (STAT6) signaling pathway activities were assessed. We found that Cy-3-g significantly inhibited OVA-induced inflammatory cell infiltration and mucus hyper-production in lung tissues, reduced the production of interleukin 4 (IL-4), interleukin 5 (IL-5) and interleukin 13 (IL-13) in BALF. Furthermore, Cy-3-g effectively suppressed OVA-induced up-regulation of the IL-4R alpha-STAT6 signaling pathway activity of the lung tissues. These results demonstrated that dietary Cy-3-g could attenuate allergic airway inflammation in a murine asthma model, and Cy-3-g might be used as an agent for asthma prevention and/or treatment in the future.