Cell cycle analysis of budding yeast using SYTOX Green.
Cell cycle analysis of budding yeast using SYTOX Green.
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DOI：
10.1002/0471142956.cy0723s26
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发表时间：
2004-11-01
影响因子：
--
通讯作者：
Haase, Steven B
中科院分区：
文献类型：
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作者：
Haase, Steven B
DNA含量的流式细胞术测定是芽殖酵母（Saccharomyces cerevisiae）细胞周期分析的标准工具。在标准乙醇固定方案中使用SYTOX绿色代替碘化丙啶（PI）可提高细胞周期分析的准确性和重现性。SYTOX染色的细胞表现出改善的变异系数（CV），DNA含量和荧光之间的更好的相关性，并在实验条件下，与PI染色的细胞相比，变化的敏感性降低。SYTOX绿色协议为研究人员提供了一个更准确和可靠的替代标准PI为基础的协议，用于分析芽殖酵母细胞周期。
Flow cytometric determination of DNA content is a standard tool for cell cycle analysis in the budding yeast, Saccharomyces cerevisiae. Using SYTOX Green instead of propidium iodide (PI) in a standard ethanol fixation protocol increases the accuracy and reproducibility of cell cycle analyses. SYTOX-stained cells exhibit improved coefficients of variation (CVs), a better correlation between DNA content and fluorescence, and decreased sensitivity to variances in experimental conditions when compared with PI-stained cells. The SYTOX Green protocol provides researchers with a more accurate and reliable alternative to standard PI-based protocols for the analysis of the budding yeast cell cycle.