Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
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细胞外囊泡研究的最低信息标准2018版（MISEV2018）：国际细胞外囊泡协会立场声明及MISEV2014指南更新
DOI：
10.1080/20013078.2018.1535750
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发表时间：
2018-11-23
影响因子：
16
通讯作者：
Zuba-Surma, Ewa K.
中科院分区：
文献类型：
--
作者：
Thery, Clotilde;Witwer, Kenneth W.;Zuba-Surma, Ewa K.
在过去的十年中，描述细胞外囊泡（EV）的生理和病理功能的科学出版物的数量急剧增加，细胞外囊泡是一个涵盖细胞释放的膜结构的各种亚型的集合术语，称为外泌体、微囊泡、微粒、核外体、癌小体、凋亡小体和许多其他名称。然而，当与这些实体一起工作时会出现特定的问题，其大小和数量通常使它们难以获得相对纯的制剂，并且难以适当地表征。国际细胞外囊泡学会（ISEV）于2014年提出了细胞外囊泡研究的最小信息（“MISEV”）指南。我们现在根据过去四年来集体知识的演变更新这些“MISEV 2014”指南。需要考虑的一个重要问题是，将特定功能归于一般EV或EV亚型，需要报告特定信息，而不仅仅是描述粗品、潜在污染和异质制剂中的功能。例如，与其他生物药理学上类似的EV相比，外泌体被赋予精致和特异性活性的说法仍然难以得到实验支持，因为我们对其生物发生和释放的特定分子机制的知识仍然有限。MISEV 2018指南包括建议的协议和步骤的表格和概述，以记录特定的EV相关功能活动。最后，提供了一份清单，其中概述了要点。
The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.