Localized Rac activation dynamics visualized in living cells
Localized Rac activation dynamics visualized in living cells
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DOI：
10.1126/science.290.5490.333
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发表时间：
2000-10-13
期刊：
影响因子：
56.9
通讯作者：
Hahn, KM
中科院分区：
文献类型：
--
作者：
Kraynov, VS;Chamberlain, C;Hahn, KM
信号蛋白被认为在空间和时间上受到严格调控，以产生特异性和局部效应。对于Rac和其他小的鸟苷三磷酸酶，与鸟苷三磷酸的结合导致与下游靶标的相互作用并调节亚细胞定位。开发了一种称为FLAIR（Rho蛋白的荧光激活指示剂）的方法来量化活细胞中Rad核苷酸状态的时空动态。FLAIR显示精确的空间控制生长因子诱导的Rac激活，在膜皱褶和在运动细胞的前沿激活的梯度。FLAIR证实了一种普遍适用的方法，用于检查蛋白质活性的时空控制。
Signaling proteins are thought to be tightly regulated spatially and temporally in order to generate specific and Localized effects. For Rac and other small guanosine triphosphatases, binding to guanosine triphosphate Leads to interaction with downstream targets and regulates subcellular localization. A method called FLAIR (fluorescence activation indicator for Rho proteins) was developed to quantify the spatio-temporal dynamics of the Rad nucleotide state in Living cells. FLAIR revealed precise spatial control of growth factor-induced Rac activation, in membrane ruffles and in a gradient of activation at the leading edge of motile cells. FLAIR exemplifies a generally applicable approach for examining spatio-temporal control of protein activity.