R3hdml regulates satellite cell proliferation and differentiation
R3hdml regulates satellite cell proliferation and differentiation
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DOI：
10.15252/embr.201947957
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发表时间：
2019-11
期刊：
影响因子：
7.7
通讯作者：
Kenichi Sakamoto;Y. Furuichi;Masashi Yamamoto;Megumi Takahashi;Y. Akimoto;T. Ishikawa;Takahiko Shimizu;M. Fujimoto;Aki Takada‐Watanabe;Aiko Hayashi;Yoshitaka Mita;Y. Manabe;N. Fujii;Ryoichi Ishibashi;Y. Maezawa;C. Betsholtz;K. Yokote;M. Takemoto
中科院分区：
文献类型：
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作者：
Kenichi Sakamoto;Y. Furuichi;Masashi Yamamoto;Megumi Takahashi;Y. Akimoto;T. Ishikawa;Takahiko Shimizu;M. Fujimoto;Aki Takada‐Watanabe;Aiko Hayashi;Yoshitaka Mita;Y. Manabe;N. Fujii;Ryoichi Ishibashi;Y. Maezawa;C. Betsholtz;K. Yokote;M. Takemoto
在这项研究中，我们发现了一种以前未被表征的骨骼卫星细胞分泌蛋白，R3h结构域含有-样（R3hdml）。R3hdml在小鼠骨骼肌发育和分化过程中表达增加。R3hdml基因敲除（KO）小鼠的体重和骨骼肌质量低于对照组小鼠。与对照小鼠相比，R3hdml KO小鼠骨骼肌中细胞周期相关标志物、Akt磷酸化和胰岛素样生长因子的表达水平降低。心肌毒素（CTX）诱导的肌肉损伤在肌肉再生过程中R3hdml的表达增加。注射CTX后，R3hdml KO小鼠的握力恢复明显受损，R3hdml可使其恢复。我们的研究结果表明，R3hdml是骨骼肌发育、再生，特别是卫星细胞增殖和分化所必需的。
In this study, we identified a previously uncharacterized skeletal satellite cell‐secreted protein, R3h domain containing‐like (R3hdml). Expression of R3hdml increases during skeletal muscle development and differentiation in mice. Body weight and skeletal muscle mass of R3hdml knockout (KO) mice are lower compared to control mice. Expression levels of cell cycle‐related markers, phosphorylation of Akt, and expression of insulin‐like growth factor within the skeletal muscle are reduced in R3hdml KO mice compared to control mice. Expression of R3hdml increases during muscle regeneration in response to cardiotoxin (CTX)‐induced muscle injury. Recovery of handgrip strength after CTX injection was significantly impaired in R3hdml KO mice, which is rescued by R3hdml. Our results indicate that R3hdml is required for skeletal muscle development, regeneration, and, in particular, satellite cell proliferation and differentiation.