The three-dimensional structure of human interphase chromosomes is related to the transcriptome map
The three-dimensional structure of human interphase chromosomes is related to the transcriptome map
复制标题
DOI：
10.1128/mcb.00208-07
复制
发表时间：
2007-06-01
影响因子：
5.3
通讯作者：
van Driel, Roel
中科院分区：
文献类型：
--
作者：
Goetze, Sandra;Mateos-Langerak, Julio;van Driel, Roel
人类间期核中染色体纤维的三维（3D）组织是基因调控的一个重要但知之甚少的方面。在这里，我们定量分析和比较的3D结构的两种类型的基因组结构域所定义的人类转录组图谱。虽然脊是基因密集的，并显示高表达水平，反脊，另一方面，是基因穷人和携带基因表达水平低。我们发现，脊一般不太凝聚，更不规则的形状，位于更接近核中心比antiridges。显示不同基因表达模式和核型的六种人类细胞系共享染色质的这些结构参数。这表明这两种类型的基因组结构域的染色质结构在很大程度上独立于基因表达和分化状态的组织特异性变化。此外，我们表明，有显着很少的混合染色质从同一染色体的不同部分在一个染色体领土，既不是从相邻的，也不是从遥远的部分。这表明，染色体纤维具有紧凑的结构，空间抑制混合。总之，我们的研究结果揭示了与基因组结构和功能相关的3D染色体结构的新的一般方面。
The three-dimensional (3D) organization of the chromosomal fiber in the human interphase nucleus is an important but poorly understood aspect of gene regulation. Here we quantitatively analyze and compare the 3D structures of two types of genomic domains as defined by the human transcriptome map. While ridges are gene dense and show high expression levels, antiridges, on the other hand, are gene poor and carry genes that are expressed at low levels. We show that ridges are in general less condensed, more irregularly shaped, and located more closely to the nuclear center than antiridges. Six human cell lines that display different gene expression patterns and karyotypes share these structural parameters of chromatin. This shows that the chromatin structures of these two types of genomic domains are largely independent of tissue-specific variations in gene expression and differentiation state. Moreover, we show that there is remarkably little intermingling of chromatin from different parts of the same chromosome in a chromosome territory, neither from adjacent nor from distant parts. This suggests that the chromosomal fiber has a compact structure that sterically suppresses intermingling. Together, our results reveal novel general aspects of 3D chromosome architecture that are related to genome structure and function.