Mechanism of Hydrogen Peroxide Formation by Spermatozoa and the Role of Amino-Acids in Sperm Motility
Mechanism of Hydrogen Peroxide Formation by Spermatozoa and the Role of Amino-Acids in Sperm Motility
复制标题
精子形成过氧化氢的机制以及氨基酸在精子活力中的作用
作者：
J. Tošić
已经证明，用新鲜射出的牛精液稀释蛋黄介质，精子在有氧条件下会产生一种抑制精子呼吸的物质。这种抑制剂后来被化学鉴定为过氧化氢，并被证明是由于精子对蛋黄及其可透析部分中存在的一种物质的代谢活动而形成的。从那时起，对蛋黄物质进行了进一步的纯化和鉴定；现在已经证明，过氧化氢是由分离的物质的精子氧化脱氨形成的，这种物质可以被L-色氨酸、L-苯丙氨酸和L-酪氨酸取代，但不被许多其他被测试的氨基酸取代。由于上述反应而产生的抑制作用不仅可以通过其对耗氧量的影响来检测，还可以通过其对精子活力的降低来检测。
IT has been demonstrated1, using freshly ejaculated bovine semen diluted with egg-yolk medium, that spermatozoa produce under aerobic conditions an inhibitor of sperm respiration. The inhibitor was afterwards identified chemically2 as hydrogen peroxide, and was shown to be formed as the result of metabolic activity of spermatozoa upon a substance present in the egg-yolk and its dialysable portion. Since then, further purification and characterization of the egg-yolk substance have been carried out; and it has now been demonstrated that hydrogen peroxide is formed as the result of an oxidative deamination by spermatozoa of the substance isolated, which is replaceable, with the same effect, only by l-tryptophane, l-phenylalanine and l-tyrosine, but not by any of many other amino-acids tested. The inhibition which develops as the result of the above reaction can be detected not only by its effect upon oxygen consumption but also by its effect upon the reduction of motility of spermatozoa.