Analysis of binding of a technetium-99m-labeled monoclonal antibody to lentivirus-infected cells.
Analysis of binding of a technetium-99m-labeled monoclonal antibody to lentivirus-infected cells.
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分析锝-99m 标记的单克隆抗体与慢病毒感染细胞的结合。
DOI：
10.1097/00004424-199011000-00006
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发表时间：
1990
影响因子：
6.7
通讯作者：
Sande,RD
中科院分区：
文献类型：
--
作者：
Papageorges,M;Gavin,PR;Adams,DS;Cheevers,WP;Barbee,DD;Sande,RD
山羊关节炎-脑炎(CAE)是研究慢病毒感染的模型。作者的假设是，放射免疫检测有可能检测到感染细胞表面的慢病毒蛋白。用~(99m)Tc标记CAE病毒(CAEV)相关糖蛋白的单抗(CAEV92A1)和对照抗体。用细胞结合试验评价99mTc标记抗体对CAEV感染细胞的免疫反应性和结合特性。99mTC-CAEV92A1优先与多聚甲醛固定的CAEV活体感染细胞结合。~(99m)Tc-CAEV92A1似乎没有从其结合部位迅速脱落。
Caprine arthritis-encephalitis (CAE) is a model for the study of lentiviral infections. The authors’ hypothesis is that radio-immunodetection has the potential to detect lentiviral proteins at the surface of infected cells. A monoclonal antibody (CAEV92A1) specific for a CAE virus (CAEV)-associated glycoprotein and a control antibody were radiolabeled with technetium-99m (99m Tc) using the pretinning method. Cell binding assays were used to evaluate immunoreactivity and binding properties of 99m Tc-labeled antibodies to CAEV-infected cells. 99m Tc-CAEV92A1 bound preferentially to paraformaldehyde-fixed and live CAEV-infected cells. 99m Tc-CAEV92A1 did not appear to be shed rapidly from its binding site.