Sequence analysis of the gene encoding alternansucrase, a sucrose glucosyltransferase from Leuconostoc mesenteroides NRRL B-1355
Sequence analysis of the gene encoding alternansucrase, a sucrose glucosyltransferase from Leuconostoc mesenteroides NRRL B-1355
复制标题
DOI：
10.1016/s0378-1097(99)00572-8
复制
发表时间：
2000-01-01
影响因子：
2.1
通讯作者：
Monsan, P
中科院分区：
文献类型：
--
作者：
Argüello-Morales, MA;Remaud-Simeon, M;Monsan, P
从肠膜明串珠菌NRRL B-1355中克隆了编码交替蔗糖酶（alternansucrase，ASR）的基因，并在大肠杆菌中表达。重组酶催化蔗糖和麦芽糖受体合成寡交替糖。从序列比较中可以看出，ASR具有与对连续α-1，3糖苷键或连续α-1，6糖苷键合成具有特异性的GTF所描述的结构域相同的结构域。然而，可变区和葡聚糖结合结构域比其他GTF长（分别长100和200个氨基酸）。N-催化结构域与L.肠膜具有三个可能参与葡糖基酶形成的决定簇。(C)2000年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
The gene encoding alternansucrase (ASR) from Leuconostoc mesenteroides NRRL B-1355, an original sucrose glucosyltransferase (GTF) specific to alternating alpha-1,3 and alpha-1,6 glucosidic bond synthesis, was cloned, sequenced and expressed into Escherichia coli. Recombinant enzyme catalyzed oligoalternan synthesis from sucrose and maltose acceptor. From sequence comparison, it appears that ASR possesses the same domains as those described for GTFs specific to either contiguous alpha-1,3 osidic bond or contiguous alpha-1,6 osidic bond synthesis. However, the variable region and the glucan binding domain are longer than in other GTFs (by 100 and 200 amino acids respectively). The N-catalytic domain which presents 49% identity with the other GTFs from L. mesenteroides possesses the three determinants potentially involved in the glucosyl enzyme formation. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.