Enhancement of antigen-presenting cell surface molecules involved in cognate interactions by immunostimulatory DNA sequences
Enhancement of antigen-presenting cell surface molecules involved in cognate interactions by immunostimulatory DNA sequences
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DOI：
10.1093/intimm/11.7.1111
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发表时间：
1999-07-01
影响因子：
4.4
通讯作者：
Raz, E
中科院分区：
文献类型：
--
作者：
Martin-Orozco, E;Kobayashi, H;Raz, E
Bacterial genomic DNA, plasmid DNA (pDNA) and synthetic oligodeoxynucleotides (ODN) containing immunostimulatory DNA sequences (ISS) have been proposed to foster a T(h)1 response via the release of type 1 cytokines from macrophages, dendritic cells, NK cells and B cells, In this study, we show that ISS-enriched DNA up-regulates a distinct profile of cell surface molecules on macrophages and B cells in vitro and in vivo. ISS-ODN and ISS-containing pDNA enhanced the expression of antigen presentation molecules (MHC class I and II), co-stimulatory molecules (B7-1, B7-2 and CD40), cytokine receptors (IFN-gamma receptor and IL-2 receptor), an adhesion molecule (ICAM-1) and an Fc receptor (Fc gamma receptor) on murine B cells or bone marrow-derived macrophages, The increased expression of these surface molecules is seen in purified cell populations and is largely independent of the effects of type 1 cytokines, Splenic antigen-presenting cells stimulated with ISS-ODN in vivo efficiently activate naive T cells and bias their differentiation toward a T(h)1 phenotype in vitro, Thus, the induction of both type 1 cytokines and a distinct profile of cell surface molecules contributes to the potent immunostimulatory effects of ISS-containing DNA on innate and adaptive immunity.
