Suppression of Arabidopsis flowering by near‐null magnetic field is mediated by auxin
Suppression of Arabidopsis flowering by near‐null magnetic field is mediated by auxin
复制标题
DOI：
10.1002/bem.22086
复制
发表时间：
2018-01
影响因子：
1.9
通讯作者：
Chunxiao Xu;Yuxia Zhang;Yang Yu;Yue Li;Shufeng Wei
中科院分区：
文献类型：
--
作者：
Chunxiao Xu;Yuxia Zhang;Yang Yu;Yue Li;Shufeng Wei
我们以前发现拟南芥的开花受到近零磁场的抑制，这与隐花色素有关。生长素在拟南芥开花过程中起着重要作用。为了验证生长素是否参与了近零磁场对拟南芥开花的抑制，我们检测了近零磁场下野生型拟南芥和隐花色素双突变体cry 1/cry 2的生长素水平以及生长素转运和信号基因的表达。我们发现，与当地地磁场对照相比，近零磁场中野生型植物根系中的吲哚-3-乙酸（IAA）水平显著增加，而近零磁场中33天龄野生型植物根系中的IAA水平显著低于对照。近零磁场可上调野生型植物中生长素转运蛋白基因PIN 1、PIN 3和PIN 7的表达。转录抑制基因IAA 1、IAA 5、IAA 6、IAA 16和IAA 19的转录水平在近零磁场的野生型植物中显著高于对照植物。然而，在近零磁场中，cry 1/cry 2突变体的IAA水平和所有检测到的基因的表达与对照相似。我们的研究结果表明，近零磁场通过上调生长素转运蛋白基因的转录水平来影响生长素在拟南芥中的分布，近零磁场下生长素分布的改变和转录抑制基因表达的增加导致拟南芥开花延迟，这是由隐花色素介导的。生物电磁学39：15-24，2018.© 2017威利期刊公司.
We previously found that flowering of Arabidopsis was suppressed by near‐null magnetic field, which was related to cryptochrome. Auxin plays an important role in Arabidopsis flowering. To test whether auxin is involved in the suppression of Arabidopsis flowering by near‐null magnetic field, we detected auxin level and expressions of auxin transport and signaling genes in wild‐type Arabidopsis plants and cryptochrome double mutant, cry1/cry2, grown in near‐null magnetic field. We found that indole‐3‐acetic acid (IAA) level in roots of wild‐type plants in near‐null magnetic field was significantly increased compared with the local geomagnetic field control, while IAA level in rosettes of 33‐day‐old wild‐type plants in near‐null magnetic field was significantly lower than the control. Expressions of three auxin transporter genes, PIN1, PIN3, and PIN7, in wild‐type plants were upregulated by near‐null magnetic field. Transcript levels of transcriptional repressor genes, IAA1, IAA5, IAA6, IAA16, and IAA19, were significantly higher in wild‐type plants in near‐null magnetic field than in control plants. However, IAA level and expressions of all the detected genes in cry1/cry2 mutants in near‐null magnetic field were similar to controls. Our results suggest that near‐null magnetic field affects the distribution of auxin in Arabidopsis by transcriptional upregulation of auxin transporter genes, and that change in distribution of auxin and increased expressions of transcriptional repressor genes result in delay of flowering in Arabidopsis in near‐null magnetic field, which are mediated by cryptochrome. Bioelectromagnetics. 39:15–24, 2018. © 2017 Wiley Periodicals, Inc.