Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy
Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy
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DOI：
10.1007/978-1-4939-6728-5_4
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发表时间：
2017-01-01
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影响因子：
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通讯作者：
Brisson, Alain R.
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文献类型：
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作者：
Linares, Romain;Tan, Sisareuth;Brisson, Alain R.
细胞外囊泡（EV）是存在于血液和其他体液中的细胞衍生的囊泡。EV因其多样的生理病理学作用和潜在的生物医学应用而引起人们的极大兴趣。然而，电动汽车的表征和量化构成了重大挑战，主要是由于其尺寸小，缺乏适合其研究的方法。电子显微镜自最初发现以来，对EV领域做出了重大贡献。在这里，我们描述了使用两个透射电子显微镜（TEM）技术的成像和定量电动汽车。Cryo-TEM结合受体特异性金标记用于揭示EV的形态，大小和表型，而它们的计数在EM网格上高速沉降后实现。
Extracellular vesicles (EVs) are cell-derived vesicles that are present in blood and other body fluids. EVs raise major interest for their diverse physiopathological roles and their potential biomedical applications. However, the characterization and quantification of EVs constitute major challenges, mainly due to their small size and the lack of methods adapted for their study. Electron microscopy has made significant contributions to the EV field since their initial discovery. Here, we describe the use of two transmission electron microscopy (TEM) techniques for imaging and quantifying EVs. Cryo-TEM combined with receptor-specific gold labeling is applied to reveal the morphology, size, and phenotype of EVs, while their enumeration is achieved after high-speed sedimentation on EM grids.