An optical method for the rapid measurement of micromolar concentrations of nitrate in marine phytoplankton cultures
An optical method for the rapid measurement of micromolar concentrations of nitrate in marine phytoplankton cultures
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DOI：
10.1023/a:1008046023487
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发表时间：
1999-04-01
影响因子：
3.3
通讯作者：
Harrison, PJ
中科院分区：
文献类型：
--
作者：
Collos, Y;Mornet, F;Harrison, PJ
根据人工海水主要成分在紫外区的吸收光谱，采用紫外吸收光谱法测定样品在单一波长(220 Nm)的吸光度，建立了测定硝酸盐的紫外吸收光谱法。不需要使用试剂，也不需要复杂的仪器。在人工海水中配制的标准物质，吸光度与硝酸盐浓度在500mmolN-L-1范围内呈线性关系，检出限为1mmolN L-1。精密度为1.5%。尿素和氨基酸在海水的典型浓度下不干扰。该方法也测量亚硝酸盐，但这种干扰只对分泌大量亚硝酸盐的物种变得重要。该方法速度极快，实施简单，不需要使用镉等有毒化学物质。这对于快速监测实验室培养的海洋浮游植物中的硝酸盐浓度应该是有用的。
This method of nitrate determination by ultraviolet absorption spectrometry is based on the measurement of sample absorbance at a single wavelength (220 nm), which was chosen on the basis of the absorption spectra of the main components of artificial seawater in the ultraviolet domain. No reagents are used and no sophisticated instruments are necessary. For standards prepared in artificial seawater, the relationship between absorbance and nitrate concentration is linear up to 500 mu mol N L-1 and the detection limit is 1 mu mol N L-1. Precision is 1.5%. Urea and amino acids did not interfere at concentrations typical of seawater. The method also measures nitrite, but this interference only becomes important for species which excrete large amounts of nitrite. The method is extremely rapid, simple to implement and does not require the use of toxic chemicals such as cadmium. It should prove useful for monitoring quickly the nitrate concentrations in laboratory cultures of marine phytoplankton.