Transient Receptor Potential vanilloid 4 ion channel in C-fibres is involved in mechanonociception of the normal and inflamed joint
Transient Receptor Potential vanilloid 4 ion channel in C-fibres is involved in mechanonociception of the normal and inflamed joint
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C 纤维中的瞬时受体电位香草酸 4 离子通道参与正常和发炎关节的机械伤害感受
DOI：
10.1038/s41598-019-47342-x
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发表时间：
2019
影响因子：
4.6
通讯作者：
Schaible H-G
中科院分区：
文献类型：
--
作者：
Richter F;Segond von Banchet G;Schaible H-G
瞬时受体电位香草蛋白4离子通道（TRPV4）是肌肉骨骼系统中重要的渗透和机械刺激传感器，也参与伤害感觉过程。在这项研究中，我们研究了TRPV4离子通道在关节疼痛中的作用。在麻醉的大鼠中，我们记录了供应膝关节内侧的机械敏感伤害性A∂-和c -纤维。膝关节关节内注射TRPV4拮抗剂RN-1734可降低正常关节c -纤维对有害机械刺激的反应以及急性炎症关节致敏c -纤维对无害和有害机械刺激的反应。RN-1734对痛觉性A∂-纤维的反应无显著影响。在关节内应用TRPV4激动剂4αPDD、GSK 1016790a和RN-1747并没有一致地改变A∂-和c -纤维对关节机械刺激的反应，也没有诱导持续的活动。我们得出结论，TRPV4离子通道参与了c -纤维对正常关节的有害机械刺激的反应，以及在关节炎症期间c -纤维对关节机械刺激的敏感性增强。
The Transient Receptor Potential vanilloid 4 ion channel (TRPV4) is an important sensor for osmotic and mechanical stimuli in the musculoskeletal system, and it is also involved in processes of nociception. In this study we investigated the putative role of TRPV4 ion channels in joint pain. In anesthetized rats we recorded from mechanosensitive nociceptive A∂- and C-fibres supplying the medial aspect of the knee joint. The intraarticular injection of the TRPV4 antagonist RN-1734 into the knee joint reduced the responses of C-fibres of the normal joint to noxious mechanical stimulation and the responses of the sensitized C-fibres of the acutely inflamed joint to innocuous and noxious mechanical stimulation. The responses of nociceptive A∂-fibres were not significantly altered by RN-1734. The intraarticular application of the TRPV4 agonists 4αPDD, GSK 1016790 A, and RN-1747 did not consistently alter the responses of A∂- and C-fibres to mechanical stimulation of the joint nor did they induce ongoing activity. We conclude that TRPV4 ion channels are involved in the responses of C-fibres to noxious mechanical stimulation of the normal joint, and in the enhanced sensitivity of C-fibres to mechanical stimulation of the joint during inflammation of the joint.
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影响因子：
--
作者：
Phan, Mimi N.;Leddy, Holly A.;Votta, Bartholomew J.;Kumar, Sanjay;Levy, Dana S.;Lipshutz, David B.;Lee, Suk Hee;Liedtke, Wolfgang;Guilak, Farshid
通讯作者：
Guilak, Farshid
影响因子：
4.8
作者：
Suzuki, M;Mizuno, A;Imai, M
通讯作者：
Imai, M
影响因子：
3.1
作者：
Krupkova, O.;Zvick, J.;Wuertz-Kozak, K.
通讯作者：
Wuertz-Kozak, K.
影响因子：
5.6
作者：
Mizoguchi, Fumitaka;Mizuno, Atsuko;Noda, Masaki
通讯作者：
Noda, Masaki
NK1受体参与伤害感受素诱导的大鼠膝关节机械敏感性调节
影响因子：
2
作者：
J. McDougall;U. Hanesch;M. Pawlak;R. Schmidt
通讯作者：
R. Schmidt