Methanol metabolism in the Asian corn borer, Ostrinia furnacalis (Guenee) (Lepidoptera: Pyralidae)
Methanol metabolism in the Asian corn borer, Ostrinia furnacalis (Guenee) (Lepidoptera: Pyralidae)
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DOI：
10.1016/j.jinsphys.2009.10.010
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发表时间：
2010-03-01
影响因子：
2.2
通讯作者：
Li, Guo-Qing
中科院分区：
文献类型：
--
作者：
Guo, Lei;Zeng, Xiang-Yong;Li, Guo-Qing
植物产生并释放大量的甲醇，特别是当被食草动物攻击时。看来食草动物可能患有甲醇中毒。本文报道了玉米螟三龄幼虫对甲醇的耐受性和代谢。当幼虫暴露于饲料中的甲醇，甲醛和甲酸72小时，估计的LC 50值分别为28.40和29毫克/克饲料。4-甲基吡唑、3-氨基-1，2，4-三氮唑和胡椒基丁醚对甲醇的毒性有增强作用，3-氨基-1，2，4-三氮唑和胡椒基丁醚对甲醛的毒性有增强作用。而磷酸三苯酯对甲醇和甲醛的协同作用不大。这些数据表明，醇脱氢酶，可能还有过氧化氢酶和细胞色素P450单加氧酶将甲醇氧化为甲醛，过氧化氢酶和细胞色素P450单加氧酶将甲醛催化为甲酸、水和二氧化碳，羧酸酯酶可能有轻微影响。几个脂肪酸甲酯（FAME）被确定从幼虫的粪便提取物已暴露于含甲醇的饮食，在对比那些无甲醇的人工饲料。体外实验表明，幼虫粗酶液可由相应的脂肪酸和甲醇合成FAME。此外，饲料中的甲醇诱导较高的酯酶活性的第一，第二和第三龄幼虫。这些结果表明，氧化代谢和非氧化代谢是部分负责甲醇消除O。furnacalis larvae. (C)2010年由Elsevier Ltd.出版
Plants produce and release large quantities of methanol, especially when attacked by herbivores. It seems that the herbivores may suffer from methanol intoxication. Here we reported the tolerance to and the metabolism of methanol by Ostrinia furnacalis third-instar larvae. When larvae were exposed to dietary methanol, formaldehyde and formic acid for 72 h, the estimated LC50 value was 28.40 and 29 mg/g diet, respectively. Toxicity of methanol was enhanced by 4-methylpyrazole, 3-amino-1,2,4-triazole and piperonyl butoxide, and toxicity of formaldehyde was increased by 3-amino-1,2,4-triazole and piperonyl butoxide. However, triphenyl phosphate had little synergistic effects on both methanol and formaldehyde. These data indicate that alcohol dehydrogenase, and probably catalase and cytochrome P450 monooxygenase oxidize methanol to formaldehyde, catalase and cytochrome P450 monooxygenase catalyze formaldehyde to formic acid, water and carbon dioxide, and carboxylesterase may have a minor effect. Several fatty acid methyl esters (FAMEs) were identified from extracts of the frass of larvae which had been exposed to a methanol-contained diet, in contrast to those on a methanol-free artificial diet. In vitro tests revealed that a crude enzyme solution from the larvae could synthesize FAMEs from corresponding fatty acids and methanol. In addition, dietary methanol induced higher esterase activities in the first-, second- and third-instar larvae. These findings demonstrate that both oxidative metabolism and non-oxidative metabolism are partially responsible for methanol elimination in O. furnacalis larvae. (C) 2010 Published by Elsevier Ltd.