Gene Expression Profiling in Lungs of Chronic Asthmatic Mice Treated with Galectin-3: Downregulation of Inflammatory and Regulatory Genes
Gene Expression Profiling in Lungs of Chronic Asthmatic Mice Treated with Galectin-3: Downregulation of Inflammatory and Regulatory Genes
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Galectin-3 治疗的慢性哮喘小鼠肺部基因表达谱：炎症和调节基因的下调
DOI：
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发表时间：
2011
影响因子：
4.6
通讯作者：
V. del Pozo
中科院分区：
文献类型：
--
作者：
E. López;M. Zafra;B. Sastre;C. Gámez;C. Lahoz;V. del Pozo
背景哮喘是一种以Th 2细胞占优势和嗜酸性粒细胞炎症为特征的疾病。细胞因子信号传导抑制因子（SOCS）蛋白充当细胞因子信号传导的负调节因子。特别是SOCS 1和SOCS 3通过控制Th 1和Th 2细胞之间的平衡在免疫应答中发挥重要作用。在先前的研究中，我们证明了使用编码半乳糖凝集素-3（Gal-3）的质粒的基因疗法治疗慢性哮喘小鼠导致Th 2过敏性炎症的改善。方法.使用微阵列的方法，这项研究endeelly评估的变化所产生的治疗Gal-3的基因治疗提供了一个良好的特点，小鼠模型的慢性气道炎症。结果通过实时RT-PCR、蛋白质印迹和免疫组化分析得到证实。结果我们确定了一组参与不同途径的基因，其表达在用Gal-3基因治疗的小鼠中协同降低/增加。我们报道了Gal-3治疗与肺中SOCS 1和SOCS 3表达的抑制之间的相关性。结论这些结果表明，Gal-3治疗后SOCS 1和3的负调节可能是过敏性疾病的一种有价值的治疗方法。
Background. Asthma is a disorder characterized by a predominance of Th2 cells and eosinophilic inflammation. Suppressors of cytokine signaling (SOCS) proteins act as negative regulators of cytokine signaling. In particular, SOCS1 and SOCS3 play an important role in immune response by controlling the balance between Th1 and Th2 cells. In a previous study, we demonstrated that treatment of chronic asthmatic mice with gene therapy using plasmid encoding galectin-3 (Gal-3) led to an improvement in Th2 allergic inflammation. Methods. Using a microarray approach, this study endeavored to evaluate the changes produced by therapeutic Gal-3 delivered by gene therapy in a well-characterized mouse model of chronic airway inflammation. Results were confirmed by real-time RT-PCR, Western blot and immunohistochemical analysis. Results. We identify a set of genes involved in different pathways whose expression is coordinately decreased/increased in mice treated with Gal-3 gene therapy. We report a correlation between Gal-3 treatment and inhibition of SOCS1 and SOCS3 expression in lungs. Conclusion. These results suggest that negative regulation of SOCS1 and 3 following Gal-3 treatment could be a valuable therapeutic approach in allergic disease.
DOI：
10.1165/rcmb.2009-0241oc
发表时间：
2010-11-01
影响因子：
6.4
作者：
McGee, Halvor S.;Stallworth, Arthur L.;Agrawal, Devendra K.
通讯作者：
Agrawal, Devendra K.
IgE 结合蛋白。
DOI：
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发表时间：
1988
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Gritzmacher,CA;Robertson,MW;Liu,FT
通讯作者：
Liu,FT
影响因子：
6
作者：
Zuberi, RI;Hsu, DK;Liu, FT
通讯作者：
Liu, FT