SERUM-PROTEINS IN HUMAN SQUAMOUS EPITHELIUM
SERUM-PROTEINS IN HUMAN SQUAMOUS EPITHELIUM
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DOI：
10.1111/j.1365-2133.1980.tb07646.x
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发表时间：
1980-01-01
影响因子：
10.3
通讯作者：
MASON, DY
中科院分区：
文献类型：
--
作者：
CORUH, G;MASON, DY
本文介绍一种新的高特异性免疫过氧化物酶染色技术（标记抗原法），用于检测人皮肤粘膜上皮细胞中的血清蛋白。大多数标本含有表现出强弥漫性细胞内染色的细胞。这些细胞通常位于上皮的中上部，通常以空泡化或核固缩为特征。双免疫酶标记（使用碱性磷酸酶和过氧化物酶）表明，不同的蛋白质通常存在于同一细胞。一种不太常见的染色形式是细胞间IG[免疫球蛋白]G的精细链。研究了来自广泛皮肤病的样本，但上皮细胞染色阳性与疾病状态之间没有明显的关系。血清蛋白可以通过被动扩散进入上皮细胞，并且注意其他情况（例如，在CNS和淋巴组织中），其中通过免疫酶染色技术观察到类似的现象。
A new immunoperoxidase staining technique of high specificity (the labeled antigen method) was used for the detection of serum proteins in the epithelium of human skin and mucous membranes. The majority of specimens contain cells showing strong diffuse intracellular staining. These cells usually lie in the upper mid-zone of the epithelium and are often charaterized by vacuolated or pyknotic nuclei. Double immunoenzymatic labeling (using alkaline phosphatase and peroxidase) showed that the different proteins are usually present in the same cells. A less frequent pattern of staining took the form of delicate strands of intercellular Ig[immunoglobin]G. Samples from a wide range of skin diseases were studied, but no clear relationship between positive epithelial staining and disease states was apparent. Serum proteins may enter epithelial cells by passive diffusion and attention is drawn to other instances (e.g., in the CNS and lymphoid tissue) where similar phenomena were observed by immunoenzymatic staining techniques.