Mitochondrial peroxiredoxin 3 is more resilient to hyperoxidation than cytoplasmic peroxiredoxins.
Mitochondrial peroxiredoxin 3 is more resilient to hyperoxidation than cytoplasmic peroxiredoxins.
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DOI：
10.1042/bj20090242
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发表时间：
2009-06-12
期刊：
影响因子：
--
通讯作者：
Hampton MB
中科院分区：
文献类型：
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作者：
Cox AG;Pearson AG;Pullar JM;Jönsson TJ;Lowther WT;Winterbourn CC;Hampton MB
Prxs（peroxiredoxins）是分解过氧化氢的半胱氨酸依赖性过氧化物酶家族。当在催化循环期间形成的次磺酸与过氧化氢反应时，PRX变得过度氧化。在本研究中，蛋白质印迹方法被开发来量化细胞中的单个2-Cys Prxs的过氧化。结果表明，Prx 1和2在低于体外数据预测的过氧化氢剂量下发生过氧化，表明细胞内因子促进过氧化。与此相反，线粒体Prx 3是相当多的抗过氧化。Jurkat T淋巴瘤细胞线粒体基质中Prx 3的浓度估计为125 μM。虽然局部细胞环境可能影响易感性，但纯化的Prx 3对过氧化也更具抗性，这表明尽管具有与敏感的真核生物Prx相似的C-末端基序，但其他结构特征必须有助于Prx 3对过氧化的先天弹性。
The Prxs (peroxiredoxins) are a family of cysteine-dependent peroxidases that decompose hydrogen peroxide. Prxs become hyperoxidized when a sulfenic acid formed during the catalytic cycle reacts with hydrogen peroxide. In the present study, Western blot methodology was developed to quantify hyperoxidation of individual 2-Cys Prxs in cells. It revealed that Prx 1 and 2 were hyperoxidized at lower doses of hydrogen peroxide than would be predicted from in vitro data, suggesting intracellular factors that promote hyperoxidation. In contrast, mitochondrial Prx 3 was considerably more resistant to hyperoxidation. The concentration of Prx 3 was estimated at 125 μM in the mitochondrial matrix of Jurkat T-lymphoma cells. Although the local cellular environment could influence susceptibility, purified Prx 3 was also more resistant to hyperoxidation, suggesting that despite having C-terminal motifs similar to sensitive eukaryote Prxs, other structural features must contribute to the innate resilience of Prx 3 to hyperoxidation.