Increased osteoclast activity in the presence of increased homocysteine concentrations
Increased osteoclast activity in the presence of increased homocysteine concentrations
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DOI：
10.1373/clinchem.2005.053363
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发表时间：
2005-12-01
影响因子：
9.3
通讯作者：
Herrmann, W
中科院分区：
文献类型：
--
作者：
Herrmann, M;Widmann, T;Herrmann, W
背景资料：血浆同型半胱氨酸（HCY）升高可能是骨质疏松性骨折的独立危险因素，因此也可能对骨代谢产生不利影响。我们分析了HCY对人破骨细胞的作用方法：我们用25 μ g/L的巨噬细胞集落刺激因子培养了17名健康男性供体（中位年龄30（5）岁）的外周血单核细胞20天（第0-11天），20 μ g/L核因子-κ B配体受体激活剂（第6-20天）和4种不同浓度的HCY（0、10、50和100 μ mol/L;第0-20天）。出于对照目的，以等摩尔浓度测试半胱氨酸和谷胱甘肽。OC被鉴定为具有抗酒石酸酸性磷酸酶（TRAP）活性和表面玻连蛋白受体的大的多核细胞。我们通过测量TRAP活性来量化OC活性。我们分析了组织蛋白酶K（CP-K）的活性在9个供体样本和估计的牙本质吸收的标准dentine-resorbing活性在3个samples.Results：经过20天的培养，大多数细胞完全分化OC。TRAP活性随HCY浓度的增加而升高（P
Background: Increased plasma homocysteine (HCY) may be an independent risk factor for osteoporotic fractures and therefore may also adversely affect bone metabolism. We analyzed the effect of HCY on human osteoclast (00 activity.Methods: We cultured peripheral blood mononuclear cells from 17 healthy male donors [median (SID) age, 30 (5) years] for 20 days with 25 mu g/L macrophagecolony stimulating factor (days 0-11), 20 mu g/L receptoractivator of nuclear factor-kappa B ligand (days 6-20), and 4 different concentrations of HCY (0, 10, 50, and 100 mu mol/L; days 0-20). For control purposes, cysteine and glutathione were tested in equimolar concentrations. OCs were identified as large, multinucleated cells with tartrate-resistant acid phosphatase (TRAP) activity and surface vitronectin receptors. We quantified OC activity by measuring TRAP activity. We analyzed cathepsin K (CP-K) activity in 9 donor samples and estimated the dentine-resorbing activity on standard dentine slices in 3 samples.Results: After 20 days of culture, most cells were fully differentiated OCs. TRAP activity increased with increasing HCY concentrations (P