Analysis of proteoglycans by high-performance liquid chromatography: a rapid micromethod for the separation of proteoglycans from tissue and cell culture.
Analysis of proteoglycans by high-performance liquid chromatography: a rapid micromethod for the separation of proteoglycans from tissue and cell culture.
复制标题
通过高效液相色谱分析蛋白多糖：一种从组织和细胞培养物中分离蛋白多糖的快速微量方法。
DOI：
10.1016/0003-2697(82)90128-2
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发表时间：
1982
影响因子：
2.9
通讯作者：
Wight,TN
中科院分区：
文献类型：
--
作者：
Iozzo,RV;Marroquin,R;Wight,TN
通过高效液相色谱法（HPLC）分离蛋白聚糖，使用两个耦合Aquapore柱，其中含有与大孔（50-100 nm）二氧化硅球共价连接的甘油丙基硅烷基团。该双柱HPLC系统可有效分离软骨蛋白聚糖聚集体和单体，而不改变其生化完整性。该系统也有效地解决了少量的同位素标记的蛋白聚糖合成培养的哺乳动物细胞。小的样品量，短的分析时间，和高重现性代表了改进的研究蛋白多糖在传统的软凝胶色谱。
Proteoglycans were separated by high-performance liquid chromatography (HPLC), using two coupled Aquapore columns containing glycerylpropylsilane groups covalently linked to large-pore (50–100 nm) silica spheres. This two-column HPLC system was effective in separating cartilage proteoglycan aggregates and monomers, without altering their biochemical integrity. This system was also effective in resolving small amounts of isotopically labeled proteoglycans synthesized by cultured mammalian cells. The small sample size, short analysis time, and high reproducibility represent improvements in the study of proteoglycans over conventional soft-gel chromatography.