Role of serotonin2A and serotonin2B/2C receptor subtypes in the control of accumbal and striatal dopamine release elicited in vivo by dorsal raphe nucleus electrical stimulation
Role of serotonin2A and serotonin2B/2C receptor subtypes in the control of accumbal and striatal dopamine release elicited in vivo by dorsal raphe nucleus electrical stimulation
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DOI：
10.1046/j.1471-4159.1999.0731033.x
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发表时间：
1999-09-01
影响因子：
4.7
通讯作者：
Spampinato, U
中科院分区：
文献类型：
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作者：
De Deurwaerdère, P;Spampinato, U
本研究利用体内微透析技术，研究了中缝背核 (DRN) 电刺激对伏隔核 (NAC) 和脑部监测的多巴胺 (DA)、3,4-二羟基苯乙酸 (DOPAC) 和 5-羟基吲哚乙酸 (5-HIAA) 细胞外水平的影响，研究了血清素 (2A) (5-HT2A) 和 5-HT2B/2C 受体的作用。氟烷麻醉大鼠的纹状体。 DRN 刺激（300 μA，1 ms，20 Hz，15 分钟）后，NAC 中的 DA 释放增强，纹状体中的 DA 释放减少。 5-HT2A 拮抗剂 SR 46349B (0.5 mg/kg) 和混合 5-HT2A/2B/2C 拮抗剂利坦色林 (0.63 mg/kg) 显着降低 DRN 刺激对 NAC 中 DA 释放的影响，但对纹状体中的 DA 释放没有影响。在任一区域中，DA 对 DRN 刺激的反应均不受 5-HT2B/2C 拮抗剂 SE 206553 (5 mg/kg) 的影响。这些化合物均不能改变 DRN 刺激在 NAC 或纹状体中诱导的 DOPAC 和 5-HIAA 流出的增强，最后，在两个脑区中，仅 SE 206553 才显着增加基础 DA 释放。这些结果表明，5-HT2A 而不是 5-HT2B/2C 受体参与内源性 5-HT 对累积 DA 释放所施加的促进控制。相反，5-HT2B/2C 受体强效抑制两个脑区的基础 DA 释放。
This study investigates, using in vivo microdialysis, the role of serotonin(2A) (5-HT2A) and 5-HT2B/2C receptors in the effect of dorsal raphe nucleus (DRN) electrical stimulation on dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), and 5-hydroxyindoleacetic acid (5-HIAA) extracellular levels monitored in the nucleus accumbens (NAC) and the striatum of halothane-anesthetized rats. Following DRN stimulation (300 mu A, 1 ms, 20 Hz, 15 min) DA release was enhanced in the NAC and reduced in the striatum. The 5-HT2A antagonist SR 46349B (0.5 mg/kg) and the mixed 5-HT2A/2B/2C antagonist ritanserin (0.63 mg/kg) significantly reduced the effect of DRN stimulation on DA release in the NAC but not in the striatum. DA responses to DRN stimulation were not affected by the 5-HT2B/2C antagonist SE 206553 (5 mg/kg) in either region. None of these compounds was able to modify the enhancement of DOPAC and 5-HIAA outflow induced by DRN stimulation in either the NAC or the striatum, Finally, in both brain regions basal DA release was significantly increased only by SE 206553, These results indicate that 5-HT2A but not 5-HT2B/2C receptors participate in the facilitatory control exerted by endogenous 5-HT on accumbal DA release. Conversely, 5-HT2B/2C receptors tonically inhibit basal DA release in both brain regions.