Evaluation of Laser Confocal Raman Spectroscopy as a Non-Invasive Method for Detecting Sperm DNA Contents.
Evaluation of Laser Confocal Raman Spectroscopy as a Non-Invasive Method for Detecting Sperm DNA Contents.
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激光共焦拉曼光谱作为一种非侵入性检测精子 DNA 方法的评价
DOI：
10.3389/fphys.2022.827941
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发表时间：
2022
影响因子：
4
通讯作者：
Hu L
中科院分区：
文献类型：
--
作者：
Li M;Ji Y;Wang D;Zhang Y;Zhang H;Tang Y;Lin G;Hu L
拉曼光谱是一种通过DNA含量差异检测精子染色体平衡状态的高效、准确的方法吗？精液样本由二倍体健康男性提供，分析参数符合现行世界卫生组织标准。通过分析从激光共聚焦拉曼光谱仪获得的相应光谱来评估DNA含量。采用荧光原位杂交技术获得精子性染色体信息。将FISH结果与拉曼光谱分析结果进行对比分析。精子头部不同部位的光谱信号强度不同，说明其化学成分不同。标准主成分分析（PCA）可以初步将不同DNA含量的精子分为两类。进一步分析表明，不同DNA含量的精子在785个DNA骨架峰和714- 1162 cm−1 DNA骨架区域上存在显著差异。X精子DNA骨架峰和区域峰均显著高于Y精子（X vs. Y，p < 0.05）。以上精子类型均经FISH证实。ROC曲线分析表明，拉曼光谱数据与FISH结果之间存在相关性。拉曼光谱可以通过分析DNA含量的差异来鉴别X和Y精子。然而，检测的准确性仍有待提高。然而，拉曼光谱在精子非整倍体检测领域具有潜在的应用价值，甚至可以在植入前遗传学检测（PGT-A）中用作精子非整倍体状态的非侵入性预测器。
Is Raman spectroscopy an efficient and accurate method to detect sperm chromosome balance state by DNA content differences? Semen samples were provided by diploid healthy men, and the analysis parameters met the current World Health Organization standards. The DNA content was assessed by analysis of the corresponding spectra obtained from a laser confocal Raman spectroscope. The sperm sex chromosome information was obtained by fluorescence in situ hybridization (FISH). Comparative analysis was performed between FISH results and Raman spectral analysis results. Different parts of the sperm head showed different spectral signal intensities, which indicated that there were different chemical components. Standard principal component analysis (PCA) can preliminarily classify sperm with different DNA contents into two groups. Further analysis showed that there were significant differences in the 785 DNA backbone peaks and 714–1,162 cm−1 DNA skeleton regions among sperm with different DNA contents. The peak and regional peak of the DNA skeleton of X sperm were significantly higher than those of Y sperm (X vs. Y, p < 0.05). The above sperm types were confirmed by FISH. ROC curve analysis shows that there is a correlation between the Raman spectrum data and FISH results. Raman spectroscopy can identify X and Y sperms by analyzing the DNA content difference. However, the accuracy of the detection still needs to be improved. Nevertheless, Raman spectroscopy has a potential application value in the field of sperm aneuploidy detection and may even be used as a non-invasive predictor of sperm aneuploid state in preimplantation genetic testing (PGT-A).
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影响因子：
6.1
作者：
Speyer, B. E.;Pizzey, A. R.;Serhal, P.
通讯作者：
Serhal, P.
影响因子：
6.7
作者：
Rodrigo, Lorena;Peinado, Vanessa;Rubio, Carmen
通讯作者：
Rubio, Carmen
影响因子：
3.7
作者：
Patassini C;Garolla A;Bottacin A;Menegazzo M;Speltra E;Foresta C;Ferlin A
通讯作者：
Ferlin A
影响因子：
1.3
作者：
Sills ES;Li X;Frederick JL;Khoury CD;Potter DA
通讯作者：
Potter DA
影响因子：
--
作者：
Quintana-Murci L;Fellous M
通讯作者：
Fellous M