Escherichia coli 83972 inhibits catheter adherence by a broad spectrum of uropathogens
Escherichia coli 83972 inhibits catheter adherence by a broad spectrum of uropathogens
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DOI：
10.1016/s0090-4295(02)02555-4
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发表时间：
2003-05-01
期刊：
影响因子：
2.1
通讯作者：
Darouiche, RO
中科院分区：
文献类型：
--
作者：
Trautner, BW;Hull, RA;Darouiche, RO
目标。导尿管表面存在非病原微生物可能会阻碍病原体在导尿管定植，从而预防尿路感染。此前，我们报道了用非致病性大肠杆菌菌株（83972）预接种导尿管可显着阻止革兰氏阳性菌（粪肠球菌）在体外的导尿管定植。为了进一步探讨这一现象，我们在体外研究了大肠杆菌 83972 是否同样可以抑制革兰氏阴性和真菌尿路病原体（普罗维登斯菌、尿路致病性大肠杆菌和白色念珠菌）的导管定植。方法。对于测试的三种尿路病原体中的每一种，我们检查了三种不同的孵育条件：（a）将大肠杆菌加尿路病原体导管暴露于大肠杆菌83972 24小时，然后暴露于尿路病原体30分钟； (b)仅将大肠杆菌导管与大肠杆菌一起孵育24小时，然后在无菌肉汤中孵育30分钟； (c)单独的尿路病原体导管在无菌肉汤中孵育24小时，然后与尿路病原体一起孵育30分钟。随后将所有导管在无菌人尿液中孵育24小时。然后冲洗导管并进行超声处理以确定每厘米粘附微生物的数量。结果。在所有情况下，将导管预先暴露于大肠杆菌 83972 均显着减少了定植于导管表面的尿路病原体的数量。结论。大肠杆菌 83972 显着阻止所有测试的细菌和真菌病原体在导管上定植。这种特殊的体外细菌干扰方法的广泛适用性需要在体内进行进一步的探索。 (C) 2003 年，爱思唯尔公司。
Objectives. The presence of a nonpathogenic organism on the surface of a urinary catheter might impede catheter colonization by pathogens and thus prevent urinary tract infection. Previously, we reported that preinoculating urinary catheters with a nonpathogenic strain of Escherichia coli (83972) significantly impeded catheter colonization in vitro by gram-positive bacteria (Enterococcus faecalis). To explore this phenomenon further, we investigated in vitro whether E. coli 83972 could likewise inhibit catheter colonization by gram-negative and fungal uropathogens (Providencia stuartii, uropathogenic E. coli, and Candida albicans).Methods. For each of the three uropathogens tested, we examined three different incubation conditions: (a) E. coli plus uropathogen catheters were exposed to E. coli 83972 for 24 hours and then to a uropathogen for 30 minutes; (b) E. coli-alone catheters were incubated with E. coli for 24 hours and then in sterile broth for 30 minutes; and (c) uropathogen-alone catheters were incubated in sterile broth for 24 hours before the 30-minute incubation with the uropathogen. All catheters were subsequently incubated in sterile human urine for 24 hours. Catheters were then rinsed and sonicated to determine the numbers of adherent organisms per centimeter.Results. Pre-exposure of the catheter to E. coli 83972 in all cases significantly reduced the number of uropathogens colonizing the catheter surfaces.Conclusions. E. coli 83972 significantly impedes catheter colonization by all tested bacterial and fungal pathogens. The broad applicability of this particular approach to bacterial interference in vitro invites further exploration in vivo. (C) 2003, Elsevier Inc.