Effect of a Lactobacillus Salivarius Probiotic on a Double-Species Streptococcus Mutans and Candida Albicans Caries Biofilm.
Effect of a Lactobacillus Salivarius Probiotic on a Double-Species Streptococcus Mutans and Candida Albicans Caries Biofilm.
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DOI：
10.3390/nu9111242
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发表时间：
2017-11-14
期刊：
影响因子：
5.9
通讯作者：
Jurczak A
中科院分区：
文献类型：
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作者：
Krzyściak W;Kościelniak D;Papież M;Vyhouskaya P;Zagórska-Świeży K;Kołodziej I;Bystrowska B;Jurczak A
本研究的目的是通过在双菌种生物膜模型中减少病原菌种类和生物膜质量来评估唾液乳杆菌的抗致龋作用。S的共存。mutans与C.白色念珠菌可导致龋齿恶化或日后复发。研究招募了59名诊断为幼儿龋齿（ECC）的儿童。根据世界卫生组织的指导方针定义儿童的牙列状况。参与者被分为初始牙釉质脱矿的儿童和显示牙本质损伤的儿童。本研究是在S. mutans和C.白色念珠菌临床菌株，分离自ECC患者的牙菌斑。研究了含唾液乳杆菌的益生菌对S. mutans和C.在体外模型中研究了白色念珠菌产生双物种生物膜的能力。基于其结晶紫染色，分析所形成/未降解生物膜的生物量。S. mutans和C.测定形成生物膜的白色念珠菌（CFU/mL，菌落形成单位/mL）。使用扫描电子显微镜（SEM）评价生物膜中的微生物形态。体外分析表明，S.变形杆菌增加了C.白念珠菌菌落数（CFU/mL）;酵母菌在其中产生的双菌种生物膜质量和菌丝形式。L. salivarius抑制C. albicans和S.变种人在益生菌的影响下，生物膜质量和S.变形链球菌; C. albicans和S. mutans与C.生物膜中的白色念珠菌菌落减少。此外，可以注意到，在添加益生菌之后，真菌没有形成致病潜力的菌丝或芽管。这些结果提示L.唾液腺可以分泌能够抑制致龋S形成的中间体。mutans和C.白念珠菌生物膜;并可能抑制真菌形态转化，从而降低致病性的C。白色念珠菌;削弱其致病潜力。需要进一步的研究来证明或反驳该制剂的长期影响，并找到预防方法。
The aim of the study was to evaluate the anti-cariogenic effects of Lactobacillus salivarius by reducing pathogenic species and biofilm mass in a double-species biofilm model. Coexistence of S. mutans with C. albicans can cause dental caries progression or recurrence of the disease in the future. Fifty-nine children with diagnosed early childhood caries (ECC) were recruited onto the study. The condition of the children’s dentition was defined according to the World Health Organization guidelines. The participants were divided into children with initial enamel demineralization and children showing dentin damage. The study was performed on the S. mutans and C. albicans clinical strains, isolated from dental plaque of patients with ECC. The effect of a probiotic containing Lactobacillus salivarius on the ability of S. mutans and C. albicans to produce a double-species biofilm was investigated in an in vitro model. The biomass of the formed/non-degraded biofilm was analyzed on the basis of its crystal violet staining. The number of colonies of S. mutans and C. albicans (CFU/mL, colony forming units/mL) forming the biofilm was determined. Microorganism morphology in the biofilm was evaluated using a scanning electron microscope (SEM). In vitro analysis demonstrated that the presence of S. mutans increased the number of C. albicans colonies (CFU/mL); the double-species biofilm mass and hyphal forms produced in it by the yeast. L. salivarius inhibited the cariogenic biofilm formation of C. albicans and S. mutans. Under the influence of the probiotic; the biofilm mass and the number of S. mutans; C. albicans and S. mutans with C. albicans colonies in the biofilm was decreased. Moreover; it can be noted that after the addition of the probiotic; fungi did not form hyphae or germ tubes of pathogenic potential. These results suggest that L. salivarius can secrete intermediates capable of inhibiting the formation of cariogenic S. mutans and C. albicans biofilm; and may inhibit fungal morphological transformation and thereby reduce the pathogenicity of C. albicans; weakening its pathogenic potential. Further research is required to prove or disprove the long-term effects of the preparation and to achieve preventive methods.