Considerations for Microscopic Imaging of Whole Organs and Animals.
Considerations for Microscopic Imaging of Whole Organs and Animals.
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整个器官和动物显微成像的注意事项。
DOI：
10.1093/micmic/ozad067.1083
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Smith,Megan
中科院分区：
文献类型：
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作者：
Watson,AlanM;Vasylieva,Iaroslavna;Smith,Megan
整个器官和动物的显微成像在科学研究中越来越重要，因为科学家们试图研究单个细胞以外的整个系统的结构。例如，神经生物学和血管生物学需要对跨越巨大生物距离的互连结构甚至单个细胞进行表征。此外，在大组织中识别稀有细胞类型的能力可以证明对许多研究领域有用。美国国立卫生研究院已经认识到这些技术的潜力，并对BRAIN Initiative和HuBMAP等项目进行了大量投资，这些项目资助了依赖于器官系统全面高分辨率成像的组件，以识别细胞群并构建全面的细胞类型图谱。幸运的是，生物学家越来越容易获得这种成像技术。例如，组织清除方法既简单又具有成本效益，使各级生物学家都能使用。组织清除技术的广泛适用性使其适用于大多数组织类型。此外，由于行业产品的增加以及使用公开可用的计划构建系统的能力，高速成像系统在大学核心设施中变得越来越普遍。如果您计划深入研究透明组织的成像，有几件事需要考虑。其中包括采用的清除方法以及将使用的成像系统。通过这些方法生成的数据量可以达到许多TB。因此，为如何可视化、分析数据并与社区共享数据做好准备是至关重要的，这通常是最困难的步骤。商业供应商和社区衍生项目为科学家提供了越来越多的选择，这些选择利用了计算和存储基础设施，并有助于使您的数据更容易访问。
Microscopic imaging of entire organs and animals is gaining significance in scientific research, as scientists seek to study the structures of entire systems beyond individual cells. Neurobiology and vascular biology, for instance, require the characterization of interconnected structures and even individual cells across vast biological distances. Moreover, the ability to identify rare cell types in large tissues can prove useful for many fields of study. The NIH has recognized the potential of such technologies by investing heavily in programs such as the BRAIN Initiative and HuBMAP, which have funded components reliant on comprehensive highresolution imaging of organ systems to identify cell populations and build comprehensive cell type atlases. Fortunately, the technologies that enable for this type of imaging are becoming increasingly accessible to biologists. Tissue clearing methods, for example, can be both simple and cost-effective, making them accessible to biologists of all levels. The broad applicability of tissue clearing techniques makes them useful for most tissue types. Additionally, high-speed imaging systems are becoming more common in university core facilities due to increased industry offerings and the ability to construct systems using openly available plans.If you plan to delve into imaging of cleared tissues, there are several things to consider. Among these are the clearing methods to employ and which imaging system (s) will be used. The amount of data generated by these methods can reach many terabytes. Thus, it is critical to be prepared for how the data will be visualized, analyzed, and shared with the community–often the most difficult steps. There are growing numbers of options available to scientists-offered by commercial vendors and communityderived projects-which leverage computational and storage infrastructure and help make your data more accessible.