Beneficial effects of dried pomegranate juice concentrated powder on ultraviolet B-induced skin photoaging in hairless mice
Beneficial effects of dried pomegranate juice concentrated powder on ultraviolet B-induced skin photoaging in hairless mice
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DOI：
10.3892/etm.2017.4626
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发表时间：
2017-08-01
影响因子：
2.7
通讯作者：
Lee, Young-Joon
中科院分区：
文献类型：
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作者：
Kang, Su-Jin;Choi, Beom-Rak;Lee, Young-Joon
本研究探讨了石榴汁浓缩粉（PCP）对无毛小鼠经15周UVB照射（每周3次; 0.18J/cm（2））后的抗衰老作用。通过皮肤水分、I型胶原和透明质酸含量以及I型胶原和透明质酸合成相关转录水平来评价皮肤保湿效果。同时评估皱纹形成和水肿评分（皮肤重量）沿着皮肤基质金属蛋白酶（MMP）-1、MMP-9和MMP-13转录水平。观察PCP对小鼠骨髓过氧化物酶（MPO）活性、白细胞介素（IL）-1 β和IL-10含量的影响。Caspase-3和裂解的聚（ADP-核糖）聚合酶（PARP）被用作表皮角质形成细胞的凋亡指数。为了确定PCP的抗氧化作用，检测了硝基酪氨酸和4-羟基壬烯醛免疫反应细胞，并测量了谷胱甘肽（GSH）含量、丙二醛水平、超氧阴离子产生量、Nox 2和GSH还原酶mRNA表达。结果表明，PCP能显著减少皮肤光老化引起的皱纹，提高皮肤含水量、I型胶原和透明质酸含量。此外，PCP处理组的IL-1 β水平低于UVB暴露对照组。UVB诱导的GSH耗竭也被PCP抑制。综上所述，目前的研究结果表明，PCP通过抗凋亡作用，MMP活性抑制和ECM（COL 1和透明质酸）合成相关的保湿，抗炎和抗氧化作用，对UVB诱导的光老化具有良好的保护作用。
The present study investigated the anti-aging effects of pomegranate juice concentrated powder (PCP) in hairless mice following 15 weeks of UVB irradiation (three times a week; 0.18J/cm(2)). Skin moisturizing effects were evaluated through skin water, collagen type I and hyaluronan contents, as well as collagen type I and hyaluronan synthesis-related transcript levels. Wrinkle formation and edema scores (skin weights) were also assessed, along with skin matrix metalloproteinase (MMP)-1,MMP-9 and MMP-13 transcript levels. To determine the anti-inflammatory effects of PCP, myeloperoxidase (MPO) activity, interleukin (IL)-1 beta and IL-10 contents were observed. Caspase-3 and cleaved poly(ADP-ribose) polymerase (PARP) were used as an apoptotic index in epidermal keratinocytes. To determine the anti-oxidative effects of PCP, nitrotyrosine and 4-hydroxynonenal immunoreactive cells were detected and glutathione (GSH) content, malondialdehyde levels, superoxide anion production, Nox2, and GSH reductase mRNA expression were all measured. The results indicated that skin wrinkles induced by photoaging were significantly reduced by PCP, whereas skin water contents, collagen type I and hyaluronan contents all increased. Furthermore, IL-1 beta levels in the PCP-treated groups were lower than those in the UVB-exposed control group. UVB-induced GSH depletion was also inhibited by PCP. Taken together, the results of the current study suggest that PCP has favorable protective effects against UVB-induced photoaging through anti-apoptotic effects, MMP activity inhibition and ECM (COL1 and hyaluronan) synthesis-related moisturizing, anti-inflammatory and anti-oxidative effects.