Structural basis for nitrous oxide generation by bacterial nitric oxide reductases
Structural basis for nitrous oxide generation by bacterial nitric oxide reductases
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DOI：
10.1098/rstb.2011.0310
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发表时间：
2012-05-05
影响因子：
6.3
通讯作者：
Hino, Tomoya
中科院分区：
文献类型：
--
作者：
Shiro, Yoshitsugu;Sugimoto, Hiroshi;Hino, Tomoya
报道了铜绿假单胞菌一氧化氮还原酶（cNOR）的晶体结构。它的整体结构类似于需氧和微需氧细胞色素氧化酶（COXs）的主要亚基，这与所有这些酶都是血红素铜氧化酶超家族成员的假设一致。然而，cNOR和考克斯之间的催化中心和催化质子的传递途径中观察到了实质性的结构差异，这应该反映在这些呼吸酶之间的功能差异中。在cNOR结构的基础上，我们提出了一种可能的反应机制，一氧化氮还原为一氧化二氮作为一个工作假设。
The crystal structure of the bacterial nitric oxide reductase (cNOR) from Pseudomonas aeruginosa is reported. Its overall structure is similar to those of the main subunit of aerobic and micro-aerobic cytochrome oxidases (COXs), in agreement with the hypothesis that all these enzymes are members of the haem-copper oxidase superfamily. However, substantial structural differences between cNOR and COX are observed in the catalytic centre and the delivery pathway of the catalytic protons, which should be reflected in functional differences between these respiratory enzymes. On the basis of the cNOR structure, we propose a possible reaction mechanism of nitric oxide reduction to nitrous oxide as a working hypothesis.