Ethanol and isopropanol trigger rapid egress of intracellular Eimeria tenella sporozoites
Ethanol and isopropanol trigger rapid egress of intracellular Eimeria tenella sporozoites
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乙醇和异丙醇触发细胞内柔嫩艾美耳球虫子孢子的快速排出
DOI：
10.1007/s00436-014-4224-y
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发表时间：
2015-02-01
影响因子：
2
通讯作者：
Suo, Xun
中科院分区：
文献类型：
--
作者：
Yan, Xinlei;Liu, Xianyong;Suo, Xun
离开宿主细胞是顶复门寄生虫如刚地弓形虫细胞内生命周期的重要步骤。这一现象引起了许多研究小组的关注。以往的研究表明，乙醇可以通过提高T细胞内Ca（2+）浓度来刺激微线体蛋白的释放。弓形虫，导致寄生虫从宿主细胞中逸出。然而，关于艾美耳球虫（家禽和家畜球虫病的病原体）的排泄信息知之甚少。在本报告中，我们研究了乙醇和异丙醇对艾美耳虫寄生虫逸出的影响。用乙醇和异丙醇处理鸡肾原代细胞培养的柔嫩艾美耳球虫子孢子，并对排出的虫体进行分析。乙醇和异丙醇可诱导E. tenella子孢子从宿主细胞。在寄生虫排出的宿主细胞中未发现实质性损伤。与新鲜分离的子孢子相比，排出的寄生虫的再侵入能力和繁殖力分别显著降低了43.4%和44.1%。我们还发现，当寄生虫在酒精处理前发育较长时间时，较少的子孢子从宿主细胞中逸出。这些结果表明体外逸出模式与T.弓形虫，促进破译的机制，出口的艾美耳虫寄生虫。
Egress from host cells is a vital step of the intracellular life cycle of apicomplexan parasites such as Toxoplasma gondii. This phenomenon has attracted attentions from many research groups. Previous studies have shown that ethanol could stimulate the release of microneme proteins by elevating intracellular Ca(2+) concentration of T. gondii, resulting in the parasite egress from host cells. However, little information about egress is known on Eimeria species, the causative agent of coccidiosis in poultry and livestock. In this report, we studied the effect of ethanol and isopropanol on the egress of eimerian parasites. Eimeria tenella sporozoites cultured in primary chicken kidney cells were treated with ethanol and isopropanol, then the egressed parasites were analyzed. Ethanol and isopropanol could induce the rapid egress of E. tenella sporozoites from host cells. No substantial damage was found in parasite-egressed host cells. Compared to the freshly isolated sporozoites, the re-invading ability and reproductivity of the egressed parasites significantly decreased by 43.4 and 44.1 % individually. We also found that fewer sporozoites egressed from host cells when the parasites developed for a longer time before the alcohol treatment. These results demonstrate an in vitro egress mode different from that of T. gondii, facilitating the deciphering of the mechanisms of egress of eimerian parasites.