DEPENDENCE OF HYPOXIC CELLULAR CALCIUM LOADING ON NA+-CA-2+ EXCHANGE
DEPENDENCE OF HYPOXIC CELLULAR CALCIUM LOADING ON NA+-CA-2+ EXCHANGE
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DOI：
10.1161/01.res.71.3.547
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发表时间：
1992-09-01
影响因子：
20.1
通讯作者：
SILVERMAN, HS
中科院分区：
文献类型：
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作者：
HAIGNEY, MCP;MIYATA, H;SILVERMAN, HS
Na+-Ca 2+交换已被证明有助于心脏中再灌注和再氧合诱导的细胞Ca 2+负荷和损伤。尽管事实上，[Na+]i和[Ca 2 +]i已被证明在缺血和缺氧期间升高，但尚不清楚缺氧期间发生的[Ca 2 +]i升高是否与复氧前通过Na+-Ca 2+交换的[Na+]i升高有关，以及这与细胞损伤的关系。用Na+敏感的新型荧光探针苯并呋喃三乙酸酯（SBFI）电刺激（0.2Hz）成年大鼠心肌细胞，使其暴露于无葡萄糖缺氧（Po 2
Na+-Ca2+ exchange has been shown to contribute to reperfusion- and reoxygenation-induced cellular Ca2+ loading and damage in the heart. Despite the fact that both [Na+]i and [Ca2+]i have been documented to rise during ischemia and hypoxia, it remains unclear whether the rise in [Ca2+]i occurring during hypoxia is linked to the rise in [Na+]i via Na+-Ca2+ exchange before reoxygenation and how this relates to cellular injury. Single electrically stimulated (0.2 Hz) adult rat cardiac myocytes loaded with Na+-sensitive benzofuran isophthalate (SBFI), the new fluorescent probe, were exposed to glucose-free hypoxia (Po2