A RAPID ELISA FOR MEASUREMENT OF ANTIBODIES TO NUCLEIC-ACID ANTIGENS USING UV-TREATED POLYSTYRENE MICROPLATES
A RAPID ELISA FOR MEASUREMENT OF ANTIBODIES TO NUCLEIC-ACID ANTIGENS USING UV-TREATED POLYSTYRENE MICROPLATES
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DOI：
10.1016/0022-1759(86)90390-x
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发表时间：
1986-01-01
影响因子：
2.2
通讯作者：
STOLLAR, BD
中科院分区：
文献类型：
--
作者：
ZOUALI, M;STOLLAR, BD
Pretreatment of polystyrene microplate wells with certain doses of UV light enhances their capacity for binding to single-stranded DNA, double stranded DNA and various synthetic polynucleotides. The use of UV-irradiated plates to immobilize nucleic acid antigens provides a simple, rapid, and specific ELISA for measuring anti-nucleic acid antibodies. The assay is at least as sensitive as the more complex method of precoating plates with poly(L-lysine). It is useful for detection of anti-DNA antibodies in sera of systemic lupus erythematosus patients, as well as in culture fluids of murine and human anti-DNA-secreting hybridomas.
