Conjugation in starformingRhizobium lupini
Conjugation in starformingRhizobium lupini
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星形形成中的缀合羽扇豆根瘤菌
DOI：
10.1007/bf01789140
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
W. Heumann
中科院分区：
文献类型：
--
作者：
W. Heumann
从羽扇豆（Lupinus luteus）根瘤中分离到一株具有高效成星能力的根瘤菌（ARhizobium）。通过用亚硝酸重复处理野生型来诱导该菌株的突变体。突变体标记有一个或两个营养缺陷型特征和不同的颜色（类胡萝卜素生产）。进行了两点、三点和四点杂交。最大重组率约为10%。对杂交结果的定量评价表明存在一个环状连锁图，重组是细胞间接合的结果，在接合过程中很可能总是整个染色体被转移。
ARhizobiumstrain which exhibits high efficiency of starformation has been isolated from the root nodules ofLupinus luteus. Mutants of this strain were induced by repeated treatment of the wild type with nitrous acid. The mutants are marked with one or two auxotrophic characters and with different colors (carotenoid production). Two-, three-, and four-point crosses were performed. The maximum recombination rate is around 10%. A quantitative evaluation of the results of the crosses indicates the existence of a circular linkage map.The recombination is the result of a conjugation between cells during which most probably always the whole chromosome is transferred.