Reconsolidation of a post-ingestive nutrient memory requires mTOR in the central amygdala
Reconsolidation of a post-ingestive nutrient memory requires mTOR in the central amygdala
复制标题
摄入后营养记忆的重新巩固需要中央杏仁核中的 mTOR
DOI：
10.1038/s41380-020-00874-5
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发表时间：
2021
影响因子：
11
通讯作者：
Li F
中科院分区：
文献类型：
--
作者：
Yan Y;Zhang L;Zhu T;Deng S;Ma B;Lv H;Shan X;Cheng H;Jiang K;Zhang T;Meng B;Mei B;Li WG;Li F
摄食行为和代谢动态平衡的中央控制被认为涉及一种形式的摄食后营养学习，独立于食物的味觉价值。然而，在这样的学习之后，还不知道大脑的哪些区域或电路被激活来检索存储的记忆，以及这种记忆在通过检索重新激活后是否经历了依赖于蛋白质合成的重新巩固。在本研究中，通过将味道与胃内注射葡萄糖联系起来以最小化对食物味道的评价，使用条件味道偏好范式，我们表明，摄取摄入后营养条件味道记忆可以刺激小鼠的多个大脑区域，包括杏仁中央核(CEA)。此外，记忆恢复激活了CEA中的哺乳动物靶标雷帕霉素复合体1(MTORC1)，而在恢复后立即对mTORC1进行位置特异性或全身性抑制，阻止了随后摄取后营养相关味道记忆的表达，导致了对恢复的长期抑制。综上所述，我们的发现表明，摄入后营养记忆的重新巩固过程调节了食物偏好。
The central control of feeding behavior and metabolic homeostasis has been proposed to involve a form of post-ingestive nutrient learning independent of the gustatory value of food. However, after such learning, it is unknown which brain regions or circuits are activated to retrieve the stored memory and whether this memory undergoes reconsolidation that depends on protein synthesis after its reactivation through retrieval. In the present study, using a conditioned-flavor-preference paradigm by associating flavors with intra-gastric infusion of glucose to minimize the evaluation of the taste of food, we show that retrieval of the post-ingestive nutrient-conditioned flavor memory stimulates multiple brain regions in mice, including the central nucleus of the amygdala (CeA). Moreover, memory retrieval activated the mammalian target of rapamycin complex 1 (mTORC1) in the CeA, while site-specific or systemic inhibition of mTORC1 immediately after retrieval prevented the subsequent expression of the post-ingestive nutrient-associated flavor memory, leading to a long-lasting suppression of reinstatement. Taken together, our findings suggest that the reconsolidation process of a post-ingestive nutrient memory modulates food preferences.