Introduction to mass spectrometry-based proteomics.
Introduction to mass spectrometry-based proteomics.
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DOI：
10.1007/978-1-62703-392-3_1
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Bunkenborg, Jakob
中科院分区：
文献类型：
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作者：
Matthiesen, Rune;Bunkenborg, Jakob
质谱技术已被广泛应用于生物分子的研究，其中一个快速发展的领域是蛋白质的整体分析，即蛋白质组学。理解和处理质谱数据是一项多方面的任务，需要做出许多决定，以便从实验中获得最全面的信息。本书后面的章节将深入讨论这一过程的各个方面，以及如何将不同的工具应用于许多分析挑战。这一介绍性章节的目的是作为一个基本的介绍质谱（MS）为基础的蛋白质组学设置场景的新人，并提供参考资料的指针。质谱在蛋白质组学中有许多应用，每种应用都与一些分析选择，仪器限制和数据处理步骤有关，这些步骤取决于研究的目的和进行的方法。通过选择样品制备，MS仪器和数据处理的正确组合，可以探索蛋白质组学的不同方面。本章将概述一些常用的设置和一些关键概念，其中许多概念将在后面的章节中进行更深入的探讨。
Mass spectrometry has been widely applied to study biomolecules and one rapidly developing field is the global analysis of proteins, proteomics. Understanding and handling mass spectrometry data is a multifaceted task that requires many decisions to be made to get the most comprehensive information from an experiment. Later chapters in this book deal in-depth with various aspects of the process and how different tools can be applied to the many analytical challenges. This introductory chapter is intended as a basic introduction to mass spectrometry (MS)-based proteomics to set the scene for newcomers and give pointers to reference material. There are many applications of mass spectrometry in proteomics and each application is associated with some analytical choices, instrumental limitations and data processing steps that depend on the aim of the study and means of conducting it. Different aspects of the proteome can be explored by choosing the right combination of sample preparation, MS instrumentation and data processing. This chapter gives an outline for some of these commonly used setups and some of the key concepts, many of which are explored in greater depth in later chapters.