Live imaging of intracellular pH in planarians using the ratiometric fluorescent dye SNARF-5F-AM.
Live imaging of intracellular pH in planarians using the ratiometric fluorescent dye SNARF-5F-AM.
复制标题
使用比例荧光染料 SNARF-5F-AM 对涡虫细胞内 pH 值进行实时成像。
DOI：
10.1093/biomethods/bpz005
复制
发表时间：
2019
影响因子：
3.6
通讯作者：
Levin,Michael
中科院分区：
文献类型：
--
作者：
Beane,WendyScott;Adams,DanySpencer;Morokuma,Junji;Levin,Michael
生理参数，如静息电位和pH值越来越多地被认为是细胞活性和再生，发育和癌症中组织水平事件的重要调节剂。荧光报告染料的可用性大大提高了在体内追踪这些特性的能力。Planarian扁形虫是一个重要的和高度听话的模型系统再生，干细胞生物学和神经科学，但是，没有协议已公布调查pH值在这个系统中。在这里，我们报告了一个简单而有效的协议，成像pH梯度在生活真涡虫适合完整和再生扁形虫。
Physiological parameters such as resting potential and pH are increasingly recognized as important regulators of cell activity and tissue-level events in regeneration, development, and cancer. The availability of fluorescent reporter dyes has greatly increased the ability to track these propertiesin vivo. The planarian flatworm is an important and highly tractable model system for regeneration, stem cell biology, and neuroscience; however, no protocols have been published for investigating pH in this system. Here, we report a simple and effective protocol for imaging pH gradients in living planaria suitable for intact and regenerating flatworms.