Microcomputed tomography-based structural analysis of various bone tissue regeneration models
Microcomputed tomography-based structural analysis of various bone tissue regeneration models
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DOI：
10.1038/nprot.2010.180
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发表时间：
2011-01-01
期刊：
影响因子：
14.8
通讯作者：
Gazit, Dan
中科院分区：
文献类型：
--
作者：
Kallai, Ilan;Mizrahi, Olga;Gazit, Dan
微计算机断层扫描 (microCT) 分析是评估骨组织的强大工具，因为它提供了对骨 3D 微结构的了解。它对于再生医学来说是无价的，因为它为研究人员提供了在体内和离体探索骨骼系统的机会。宏观结构特征和微观结构特征的定量评估可以提高我们估计新形成骨的质量的能力。我们开发了一种独特的程序来分析 microCT 扫描数据，以评估骨骼结构和修复。该协议描述了三种主要类型的小鼠骨再生模型（骨形成间充质干细胞的异位管理，以及长骨缺损和颅骨节段骨缺损后细胞的管理）的微CT分析程序，可以轻松适应各种其他模型。精确的协议至关重要，因为该系统对用户极其敏感，如果不应用标准化方法，结果很容易出现偏差。建议的方案每个样本需要 1.5-3.5 小时，具体取决于骨组织样本大小、使用的设备类型、扫描方案的变量和操作员的经验。
Microcomputed tomography (microCT) analysis is a powerful tool for the evaluation of bone tissue because it provides access to the 3D microarchitecture of the bone. It is invaluable for regenerative medicine as it provides the researcher with the opportunity to explore the skeletal system both in vivo and ex vivo. The quantitative assessment of macrostructural characteristics and microstructural features may improve our ability to estimate the quality of newly formed bone. We have developed a unique procedure for analyzing data from microCT scans to evaluate bone structure and repair. This protocol describes the procedures for microCT analysis of three main types of mouse bone regeneration models (ectopic administration of bone-forming mesenchymal stem cells, and administration of cells after both long bone defects and cranial segmental bone defects) that can be easily adapted for a variety of other models. Precise protocols are crucial because the system is extremely user sensitive and results can be easily biased if standardized methods are not applied. The suggested protocol takes 1.5-3.5 h per sample, depending on bone tissue sample size, the type of equipment used, variables of the scanning protocol and the operator's experience.