First Attempt To Validate Human IgG Antibody Response to Nterm-34kDa Salivary Peptide as Biomarker for Evaluating Exposure to Aedes aegypti Bites
First Attempt To Validate Human IgG Antibody Response to Nterm-34kDa Salivary Peptide as Biomarker for Evaluating Exposure to Aedes aegypti Bites
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DOI：
10.1371/journal.pntd.0001905
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发表时间：
2012-11-01
影响因子：
3.8
通讯作者：
Remoue, Franck
中科院分区：
文献类型：
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作者：
Ndille, Emmanuel Elanga;Doucoure, Souleymane;Remoue, Franck
背景：正在致力于开发新的指标，以评估人类接触伊蚊叮咬和虫媒病毒传播的风险。人类抗体(Ab)对蚊子唾液成分的反应可能是一种很有前途的评估人与媒介接触的工具。方法/主要发现：为了开发人类接触埃及伊蚊的特异性生物标志物，我们测定了对Ae的抗体反应。贝宁南部(西非)7个村庄暴露儿童中的埃及伊蚊NTerm-34 kDa唾液肽。结果表明，村与村之间的特异性免疫球蛋白反应具有高度的个体间异质性。免疫球蛋白应答与降雨量有关，从干旱(低暴露期)到雨季(高暴露期)，免疫球蛋白水平升高。提示抗NTerm-34 kDa唾液肽免疫球蛋白抗体可作为检测人群Ae暴露变异的可靠生物标志物。埃及伊蚊叮咬。结论/意义：这项初步研究强调了对这种唾液肽的抗体反应用于评估人类暴露于Ae的潜在用途。埃及伊蚊。这一生物标志物可能成为评估虫媒病毒传播风险和评估媒介控制干预措施效果的新的有前途的工具。
Background: Much effort is being devoted for developing new indicators to evaluate the human exposure to Aedes mosquito bites and the risk of arbovirus transmission. Human antibody (Ab) responses to mosquito salivary components could represent a promising tool for evaluating the human-vector contact.Methodology/Principal findings: To develop a specific biomarker of human exposure to Aedes aegypti bites, we measured IgG Ab response to Ae. aegypti Nterm-34 kDa salivary peptide in exposed children in 7 villages of Southern Benin (West Africa). Results showed that specific IgG response presented high inter-individual heterogeneity between villages. IgG response was associated with rainfall and IgG level increased from dry (low exposure) to rainy (high exposure) seasons. These findings indicate that IgG Ab to Nterm-34 kDa salivary peptide may represent a reliable biomarker to detect variation in human exposure to Ae. aegypti bites.Conclusion/Significance: This preliminary study highlights the potential use of Ab response to this salivary peptide for evaluating human exposure to Ae. aegypti. This biomarker could represent a new promising tool for assessing the risk of arbovirus transmission and for evaluating the efficacy of vector control interventions.