Foldamers with hybrid biological and synthetic sequences as selective DNA fluorescent probes.
Foldamers with hybrid biological and synthetic sequences as selective DNA fluorescent probes.
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具有混合生物和合成序列的折叠体作为选择性 DNA 荧光探针。
DOI：
10.1021/bi050945z
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发表时间：
2005
期刊：
影响因子：
2.9
通讯作者：
Li,AlexanderDQ
中科院分区：
文献类型：
--
作者：
Wang,Wei;Wan,Wei;Stachiw,Andrew;Li,AlexanderDQ
具有交替的单链脱氧核糖核酸（ssDNA）和平面荧光有机发色团的可折叠聚合物可以自组织成折叠的纳米结构，因此是具有生物序列和合成性质的混合折叠体。生物序列提供高度特异性的分子识别特性，而合成发色团的物理特性提供灵敏的荧光检测。在本文中，我们描述了合理设计的杂交折叠体在多核苷酸检测中表现出的潜力。在严格控制的实验室条件下，荧光测量表明，由于多核苷酸与一个或两个错配碱基的结合而引起的构型变化可以容易地区分。这些结果揭示了具有致动器和传感特性的纳米结构折叠体的设计和构建，这可能会发现作为生物探针的重要应用。
Foldable polymers with alternating single-strand deoxyribonucleic acid (ssDNA) and planar fluorescent organic chromophores can self-organize into folded nanostructures and hence are hybrid foldamers with biological sequences and synthetic properties. The biological sequence provides highly specific molecular recognition properties, while the physical properties of synthetic chromophores offer sensitive fluorescence detection. In this paper, we describe that rational designed hybrid foldamers exhibit potential in the detection of polynucleotides. Under strictly controlled laboratory conditions, fluorescence measurements indicate that configuration change due to binding of polynucleotides with one or two mismatched bases can be readily distinguished. These results shed light on the design and construction of nanostructured foldamers with actuator and sensory properties, which may find important applications as biological probes.