MiR‐455‐3p regulates early chondrogenic differentiation via inhibiting Runx2
MiR‐455‐3p regulates early chondrogenic differentiation via inhibiting Runx2
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DOI：
10.1016/j.febslet.2015.09.032
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发表时间：
2015-11
期刊：
影响因子：
3.5
通讯作者：
Zhiqi Zhang;C. Hou;F. Meng;Xiaoyi Zhao;Ziji Zhang;Guangxin Huang;Weishen Chen;M. Fu;W. Liao
中科院分区：
文献类型：
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作者：
Zhiqi Zhang;C. Hou;F. Meng;Xiaoyi Zhao;Ziji Zhang;Guangxin Huang;Weishen Chen;M. Fu;W. Liao
miR-455 - 3p在骨髓间充质干细胞软骨形成过程中表达上调，但其在软骨形成不同阶段的作用尚不清楚。在这里，我们发现miR-455 - 3p在ATDC 5细胞中从0 d到21 d增加，但在28 d迅速下降，并且在小鼠胚胎发育中检测到类似的表达动力学。我们发现，miR-455 - 3p作为早期软骨分化的激活剂，最有可能通过抑制Runt相关转录因子2（Runx2）的表达，如荧光素酶报告基因检测所示。总之，miR-455 - 3p可能通过直接靶向Runx2激活早期软骨形成。
The expression of miR-455-3p has been shown to be up-regulated in chondrogenesis of mesenchymal stem cell, but its role in different stages during chondrogenesis remains unknown. Here, we show that miR-455-3p is increased in ATDC5 cells from 0 d to 21 d, but rapidly decreases at 28 d, and a similar expression kinetic is detected in the development of mouse embryos. We show that miR-455-3p functions as an activator for early chondrogenic differentiation, most likely by inhibiting the expression of Runt-related transcription factor 2 (Runx2) as indicated by luciferase reporter assays. In conclusion, miR-455-3p may activate early chondrogenesis by directly targeting Runx2.