Ccrl2-centred immune-related lncRNA-mRNA co-expression network revealed the local skin immune activation mechanism of moxibustion on adjuvant arthritis mice
Ccrl2-centred immune-related lncRNA-mRNA co-expression network revealed the local skin immune activation mechanism of moxibustion on adjuvant arthritis mice
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DOI：
10.1016/j.lfs.2023.121910
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发表时间：
2023-07-14
期刊：
影响因子：
6.1
通讯作者：
Yin，Haiyan
中科院分区：
文献类型：
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作者：
Li，Yifan;Yang，Peng;Yin，Haiyan
艾灸是一种重要的传统医学外治法，作用于皮肤上的某些穴位，通常用于治疗免疫相关疾病。然而，艾灸的作用机制是否与皮肤的免疫功能，尤其是免疫相关的lncRNAs有关，目前尚不清楚。方法用完全弗氏佐剂(CFA)右后爪注射诱导佐剂性关节炎。艾灸足三里穴治疗3 周。通过足体积和血清细胞因子浓度的变化来评价艾灸的抗炎作用。免疫荧光染色检测ST36局部皮肤CD83的表达。通过转录组RNA测序(RNA-seq)和lncRNA-mRNA网络分析，构建艾灸免疫相关的lncRNA-mRNA共表达网络。结果艾灸足肿胀明显减轻足肿胀，降低血清肿瘤坏死因子-α和白介素1β浓度，显著增加足局部皮肤CD83CD83的表达。共筛选出548个差异表达的LncRNA和520个连锁的mRNAs。炎症和免疫反应是GO显著和主要的富集性术语，与炎症和免疫反应相关的主要途径包括Toll样受体、细胞因子-细胞因子受体和MAPK信号转导。免疫相关的lncRNA-mRNA共表达网络包括88个lncRNAs和36个mRNAs，CCRL2是该网络的中枢。结论艾灸可显著触发AA小鼠ST36的局部免疫激活。以CCRL2为核心的免疫相关lncRNA-mRNA共表达网络有望成为破解艾灸治疗免疫性疾病机理的新靶点。
BackgroundMoxibustion is an important external therapy of traditional medicine that operates on some acupoints on the skin and is usually used for immune-related diseases. However, whether the immune function of the skin, especially the immune-related lncRNAs, contributes to the mechanism of moxibustion remains unclear.MethodsAdjuvant arthritis (AA) was induced by injection of Complete Freund's adjuvant (CFA) into the right hind paw of mice. Moxibustion was administered on the Zusanli (ST36) acupoint for 3 weeks. The alteration of foot volume and cytokine concentration in serum was used to evaluate the anti-inflammation effect of moxibustion. CD83 expression in the local skin of ST36 was measured by immunofluorescence staining. Transcriptome RNA sequencing (RNA-seq) and lncRNA-mRNA network analysis were performed to construct a moxibustion-induced Immune-related lncRNA-mRNA co-expression network. qRT-PCR was used to validate the RNA-seq data.ResultsMoxibustion at ST36 relieved the foot swelling, decreased the TNF-α and IL-1β concentrations in serum, and obviously increased the CD83 expression at the local skin of ST36. A total of 548 differentially expressed lncRNAs and 520 linked mRNAs were screened out. The significantly and predominately enriched Go term was inflammatory and immune response, and the main pathways related to inflammatory and immune responses include Toll-like receptor, cytokine-cytokine receptor, and MAPK signaling. The immune-related lncRNA-mRNA co-expression network showed 88 lncRNAs and 36 mRNAs, and Ccrl2 is the central hub of this network.ConclusionLocal immune activation is significantly triggered by moxibustion in ST36 of AA mice. The Ccrl2-centered immune-related lncRNA-mRNA co-expression network would be a promising target for decoding the mechanism of moxibustion for immune-related diseases.