Regular production of infective sporozoites of Plasmodium falciparum and P-vivax in laboratory-bred Anopheles albimanus
Regular production of infective sporozoites of Plasmodium falciparum and P-vivax in laboratory-bred Anopheles albimanus
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DOI：
10.1080/00034983.1997.11813111
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发表时间：
1997-01-01
影响因子：
--
通讯作者：
Herrera, S
中科院分区：
文献类型：
--
作者：
Hurtado, S;Salas, ML;Herrera, S
对引起人类疟疾的寄生虫的孢子生殖周期和红细胞前疫苗的保护效力进行研究的主要限制之一是缺乏实验室饲养的按蚊作为感染性子孢子的来源。本研究的目的是在实验室中重现恶性疟原虫和间日疟原虫的生命周期，从而发展在实验室条件下定期产生这两个物种的感染性子孢子的能力。定居的白曼按蚊（布埃纳文图拉和Tecojate品系）通过喂食来自人类患者或实验接种的Aotus猴的疟原虫感染血液或体外生长的恶性疟原虫NF-54分离株的配子母细胞而感染。这些猴子被感染了哥伦比亚间日疟原虫分离株的血液阶段，然后在恢复后，被感染了来自萨尔瓦多萨尔瓦多的恶性疟原虫圣露西亚株。尽管所用的两种蚊子品系都成功地感染了两种寄生虫物种，但布埃纳文图拉蚊子品系通常比Tecojate品系更容易感染，特别是感染来自患者的寄生虫，这些患者生活在最初分离该蚊子品系的地方。猴子静脉注射蚊子体内产生的间日疟原虫子孢子后，产生了明显的有性和无性寄生虫血症;产生的配子体可以用来感染蚊子，从而产生更多的子孢子。然而，在将恶性疟原虫子孢子用于感染猴子后，实验感染未能建立。在实验室中复制间日疟原虫从人到蚊子再到猴子的完整生命周期的能力，将极大地促进许多关于间日疟和候选疟疾疫苗效力的研究。三种不同来源的恶性疟原虫孢子生殖周期的可用性也允许进行各种生物学研究。
One of the major constraints for studies on the sporogonic cycle of the parasites causing human malaria, and on the protective efficacy of pre-erythrocytic vaccines, is the scarcity of laboratory-reared Anopheles mosquitoes as a source of infective sporozoites. The aim of the present study was to reproduce the life-cycles of Plasmodium falciparum and P. vivax in the laboratory and so develop the ability to produce infective sporozoites of these two species regularly under laboratory conditions. Colonized Anopheles albimanus, of Buenaventura and Tecojate strains, were infected by feeding either on Plasmodium-infected blood, from human patients or experimentally inoculated Aotus monkeys, or on gametocytes of the P. falciparum NF-54 isolate grown in vitro. The monkeys were infected with the blood stages of a Colombian P. vivax isolate and then, after recovery, with the Santa Lucia strain of P. falciparum from El Salvador. Although both of the mosquito strains used were successfully infected with both parasite species, the Buenaventura strain of mosquito was generally more susceptible to infection than the Tecojate strain, and particularly to infection with the parasites from the patients, who lived where this strain of mosquitoes was originally isolated. Monkeys injected intravenously with the P. vivax sporozoites produced in the mosquitoes developed patent sexual and asexual parasitaemias; the gametocytes that developed could then be used to infect mosquitoes, allowing the development of more sporozoites. However, experimental infections failed to establish after the P. falciparum sporozoites were used to inoculate monkeys. The ability to reproduce the complete life cycle of P. vivax in the laboratory, from human to mosquito and then to monkey, should greatly facilitate many studies on vivax malaria and on the efficacy of candidate malaria vaccines. The availability of the sporogonic cycles of P. falciparum from three different sources should also permit a variety of biological studies.