Rhizobium-mediated induction of phenolics and plant growth promotion in rice (Oryza sativa L.)
Rhizobium-mediated induction of phenolics and plant growth promotion in rice (Oryza sativa L.)
复制标题
DOI：
10.1007/s00284-005-0296-3
复制
发表时间：
2006-05-01
影响因子：
2.6
通讯作者：
Maurya, S
中科院分区：
文献类型：
--
作者：
Mishra, RPN;Singh, RK;Maurya, S
采用反相高效液相色谱法（RP-HPLC）对接种根瘤菌RRE 6（Rhizobium leguminosarum bv. phaseoli）和ANU 843（R.豆科植物trifolii）和立枯丝核菌（Rhizoctonia solani）的侵染。根据其保留时间，在HPLC分析中检测到的主要酚酸是没食子酸、鞣酸、阿魏酸和肉桂酸。此外，在所有接种根瘤菌的水稻植株中，酚类化合物的合成比对照（未接种的植株）更一致地增强，其中在接种RRE 6和感染R.索拉尼在病原胁迫下，RRE 6表现更好，因为与用ANU 843处理的植物相比，诱导了相对较高量的酚类物质。酚酸介导诱导的系统抗性，并在病原胁迫期间为植物提供生物保护。此外，这两种根瘤菌菌株在温室条件下促进水稻植物的生长和生产力。本研究以非豆科植物（如水稻）为例，探讨了根瘤菌介导的防御反应和促生长作用，为植物-微生物共生互作提供了一个新的范例。
Qualitative and quantitative estimation of phenolic compounds was done through reverse phase-high performance liquid chromatography (RP-HPLC) from different parts (leaf, stem, and root) of rice plants after inoculation with two rhizobial strains, RRE6 (Rhizobium leguminosarum bv. phaseoli) and ANU 843 (R. leguminosarum bv. trifolii) and infection by Rhizoctonia solani. On the basis of their retention time, the major phenolic acids detected in HPLC analysis were gallic, tannic, ferulic, and cinnamic acids. Furthermore, in all Rhizobium-inoculated rice plants, synthesis of phenolic compounds was more consistently enhanced than in control (uninoculated plants), where the maximum accumulation of phenolic compounds was observed in plants inoculated with RRE6 and infection with R. solani. Under pathogenic stress, RRE6 performed better because a relatively higher amount of phenolics was induced as compared with plants treated with ANU 843. Phenolic acids mediate induced systemic resistance and provide bioprotection to plants during pathogenic stresses. In addition, both rhizobial strains promote growth and productivity of rice plants in greenhouse conditions. This report on Rhizobium-mediated defense responses and growth promotion of nonlegume (such as rice) provides a novel paradigm of symbiotic plant-microbe interaction.