Assisted fertilization by zona drilling: a mouse model for correction of oligospermia.
Assisted fertilization by zona drilling: a mouse model for correction of oligospermia.
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透明带钻孔辅助受精：用于纠正少精子症的小鼠模型。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
B. Talansky
中科院分区：
文献类型：
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作者：
J. Gordon;B. Talansky
用显微操作装置在未受精的小鼠卵母细胞透明带上制造孔。使装载有酸性台氏溶液的微针与微针表面接触，并结合机械压力使用正流动以引起微针的局部溶解。然后将处理过的卵与对照细胞进行体外受精。精子钻孔程序将实现受精所需的精子数量减少了约100倍。在透明带钻取的卵母细胞中的多精受精率不大于对照组，钻取后受精的卵母细胞，当植入假孕的寄养雌性时，以与对照组相同的速率发育至足月。结果表明，精子钻孔是一种安全、有效的提高体外受精效率的方法，在农业和医学上对精子计数低的男性赋予生育力可能是有用的。
A micromanipulation apparatus was used to produce holes in the zonae pellucidae of unfertilized mouse oocytes. A microneedle loaded with acid Tyrode's solution was brought into contact with the zona surface, and positive flow was used in conjunction with mechanical pressure to cause a localized dissolution of the zona. Treated eggs were then fertilized in vitro in comparison with control cells. The zona drilling procedure decreased the sperm count required to achieve fertilization by a factor of approximately 100. The rate of polyspermy in zona-drilled oocytes was not greater than in controls, and oocytes fertilized after drilling, when implanted into pseudopregnant foster females, developed to term at the same rate as controls. The results demonstrate that zona drilling is a safe, effective method of increasing the efficiency of fertilization in vitro and may be useful both in agriculture and medicine for conferring fertility upon males with low sperm counts.
DOI：
10.1073/pnas.77.12.7380
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
GORDON, JW;SCANGOS, GA;RUDDLE, FH
通讯作者：
RUDDLE, FH