LOCALIZATION OF INTERLEUKIN-8 IN HUMAN GINGIVAL TISSUES
LOCALIZATION OF INTERLEUKIN-8 IN HUMAN GINGIVAL TISSUES
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DOI：
10.1111/j.1399-302x.1995.tb00158.x
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发表时间：
1995-10-01
影响因子：
--
通讯作者：
KREUTZER, DL
中科院分区：
文献类型：
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作者：
FITZGERALD, JE;KREUTZER, DL
牙周炎和牙龈炎是牙周组织及其邻近组织的慢性炎症性疾病。这种部位特异性炎症的特征是多形核白细胞和T淋巴细胞的局部浸润。白细胞介素-8是一种低分子量的细胞因子，被认为是负责局部炎症的诱导和维持，我们假设，局部产生的白细胞介素-8通过调节牙龈组织中白细胞的募集和激活在慢性牙周炎中起着核心作用。为了验证这一假设，我们确定了白细胞介素-8抗原是否存在于局部，并与细胞相关。分析炎症组织和对照组织：1）白细胞介素-8抗原; 2）分子量; 3）位置; 4）白细胞介素-8的信使RNA（mRNA）。结果表明：1）IL-8抗原和mRNA在慢性炎症牙龈组织中表达升高; 2）IL-8抗原主要表达于上皮细胞层，提示IL-8可能在牙周炎中性粒细胞和T淋巴细胞的募集和活化中起重要作用。
Periodontitis and gingivitis are chronic inflammatory diseases of the periodontium and adjacent tissues. This site-specific inflammation is characterized by a local infiltration of polymorphonuclear leukocytes and T lymphocytes. Interleukin-8 is a low-molecular-weight cytokine that is thought to be responsible for the induction and maintenance of localized inflammation, We hypothesized that locally produced interleukin-8 plays a central role in chronic inflammation of periodontitis by regulating the recruitment and activation of leukocytes in the gingival tissues. To test this hypothesis, we determined whether the interleukin-8 antigen is present locally and is cell-associated. Inflamed and control tissues were analyzed: 1) for the interleukin-8 antigen; 2) by molecular weight; 3) for location; and 4) for the messenger RNA (mRNA) of interleukin-8. The conclusions from these data were that: 1) interleukin-8 antigen and mRNA was elevated in chronically inflamed gingiva; and 2) the major interleukin-8 antigen was detected only in the epithelial cell layer, These results support that interleukin-8 may play a crucial role in the recruitment and activation of neutrophils and T lymphocytes in periodontitis.