A comparative study of three cranial sensory ganglia projecting into the oral cavity: in situ hybridization analyses of neurotrophin receptors and thermosensitive cation channels
A comparative study of three cranial sensory ganglia projecting into the oral cavity: in situ hybridization analyses of neurotrophin receptors and thermosensitive cation channels
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DOI：
10.1016/s0169-328x(01)00129-2
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发表时间：
2001-09-30
期刊：
影响因子：
--
通讯作者：
Abe, K
中科院分区：
文献类型：
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作者：
Matsumoto, I;Emori, Y;Abe, K
投射到口腔的外周头颅感觉神经接受食物摄入刺激，并将感觉信号传递到中枢神经系统。为了描述和比较支配口腔的颅感觉神经节，即三叉神经节、岩神经节和膝状神经节(分别为TG、PG和GG)的特征，用核糖核酸探针进行了神经营养素受体(TrkA、TrkB和TrkC)、神经递质(P物质)和与温度感觉有关的离子通道(VR1和Trek-1)的原位杂交。在TG中，所有6个探针都产生了不同程度的阳性信号，强度和频率都不同。此外，在背根神经节中，VR1的表达与TrkA和P物质的表达有很强的相关性。在PG中，也检测到了所有六种探针的阳性信号，表达的相关性与TG显示的相似。另一方面，GG中大多数细胞对TrkB探针呈阳性反应，少数细胞对TrkC探针呈阳性反应，而其他4种探针均未观察到明显的信号。这些结果表明，TG和PG的细胞在神经营养需求和躯体感觉功能方面是不同的，而GG似乎主要由一种同质的细胞类型组成，即味觉神经元。总而言之，TG、PG和GG显示出三个神经节固有的基因表达特征。上述结果还表明，TG和PG的一部分投射出多种体感神经。这与GG和部分PG投射味觉神经的发现是一致的。(C)2001年爱思唯尔科学公司。版权所有。
Peripheral cranial sensory nerves projecting into the oral cavity receive food intake stimuli and transmit sensory signals to the central nervous system. To describe and compare the features of the cranial sensory ganglia that innervate the oral cavity, i.e., the trigeminal, petrosal, and geniculate ganglia (TG, PG, and GG, respectively), in situ hybridization was conducted using riboprobes for neurotrophin receptors (TrkA, TrkB, and TrkC), a neurotransmitter (substance P), and ion channels important for thermosensation (VR1 and TREK-1). In TG, all in six probes yielded positive signals to various extent in intensity and frequency. In addition, a strong correlation between the expression of VR1 and those of TrkA and substance P was observed as in the case of the dorsal root ganglia. In PG, positive signals to all six probes were also detected, and the correlation of expression was similar to that shown by TG. On the other hand, most cells in GG were positive to the TrkB probe, and a small number of cells were positive to the TrkC probe, but no significant signal was observed for the other four probes. These results indicate that TG and PG consist of cells that are heterogeneous in terms of neurotrophin requirement and somatosensory functions, and that GG seems to consist mainly of a homogeneous cell type, gustatory neurons. In conclusion, TG, PG, and GG, show gene expression characteristics intrinsic to the three ganglia. It is also concluded that TG and a portion of PG project several types of somatosensory nerves. This is consistent with the finding that GG and a portion of PG project gustatory nerves. (C) 2001 Elsevier Science BY. All rights reserved.